Identification of a novel cetacean polyomavirus from a common dolphin (Delphinus delphis) with Tracheobronchitis

A female short-beaked common dolphin calf was found stranded in San Diego, California in October 2010, presenting with multifocal ulcerative lesions in the trachea and bronchi. Viral particles suggestive of polyomavirus were detected by EM, and subsequently confirmed by PCR and sequencing. Full geno...

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Bibliographic Details
Published in:PloS one 2013-07, Vol.8 (7), p.e68239-e68239
Main Authors: Anthony, Simon J, St Leger, Judy A, Navarrete-Macias, Isamara, Nilson, Erica, Sanchez-Leon, Maria, Liang, Eliza, Seimon, Tracie, Jain, Komal, Karesh, William, Daszak, Peter, Briese, Thomas, Lipkin, W Ian
Format: Article
Language:English
Subjects:
Adenoviruses
Analysis
Animals
Anorexia
Antigens
Apoptosis
Aquatic mammals
Biology
Bronchi
Bronchitis
Bronchitis - diagnosis
Bronchitis - etiology
Bronchitis - veterinary
Bronchitis - virology
Bronchus
California
Cetacea
Cetacea - virology
Cladistic analysis
Common Dolphins - virology
Delphinus delphis
DNA sequencing
Dolphins
Dolphins (Mammals)
Epidemiology
Female
Gene expression
Gene sequencing
Genomes
Genomics
Health aspects
Infections
Lesions
Mammals
Marine
Marine mammals
Medicine
Morphology
Mucosa
Pathogenesis
Phylogeny
Polyomaviridae - isolation & purification
Polyomaviridae - physiology
Polyomavirus
Proteins
Public health
Respiratory diseases
Respiratory Tract Infections - diagnosis
Respiratory Tract Infections - etiology
Respiratory Tract Infections - veterinary
Respiratory Tract Infections - virology
Stranding
Trachea
Trachea - pathology
Trachea - virology
Tracheobronchitis
Veterinary Science
Virus diseases
Viruses
Zalophus californianus
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Description
Summary:A female short-beaked common dolphin calf was found stranded in San Diego, California in October 2010, presenting with multifocal ulcerative lesions in the trachea and bronchi. Viral particles suggestive of polyomavirus were detected by EM, and subsequently confirmed by PCR and sequencing. Full genome sequencing (Ion Torrent) revealed a circular dsDNA genome of 5,159 bp that was shown to form a distinct lineage within the genus Polyomavirus based on phylogenetic analysis of the early and late transcriptomes. Viral infection and distribution in laryngeal mucosa was characterised using in-situ hybridisation, and apoptosis observed in the virus-infected region. These results demonstrate that polyomaviruses can be associated with respiratory disease in cetaceans, and expand our knowledge of their diversity and clinical significance in marine mammals.
ISSN:1932-6203
1932-6203
DOI:10.1371/journal.pone.0068239