Studies on the assembly characteristics of large subunit ribosomal proteins in S. cerevisae

• Published in: PloS one 2013-07, Vol.8 (7), p.e68412-e68412
• Main Authors: Ohmayer, Uli, Gamalinda, Michael, Sauert, Martina, Ossowski, Julius, Pöll, Gisela, Linnemann, Jan, Hierlmeier, Thomas, Perez-Fernandez, Jorge, Kumcuoglu, Beril, Leger-Silvestre, Isabelle, Faubladier, Marlène, Griesenbeck, Joachim, Woolford, John, Tschochner, Herbert, Milkereit, Philipp
• Format: Article
• Language: English
• Subjects: Analysis; Assembly; Baking yeast; Biosynthesis; Chromatography, Affinity; E coli; Escherichia coli; Experiments; Laboratories; Mass Spectrometry; Mass spectroscopy; Protein composition; Protein Multimerization - physiology; Proteins; Proteome - analysis; Proteomics - methods; Ribosomal DNA; Ribosomal proteins; Ribosomal Proteins - chemistry; Ribosomal Proteins - metabolism; Ribosomal subunits; Ribosome Subunits, Large - chemistry; Ribosome Subunits, Large - metabolism; Ribosomes; RNA Precursors - analysis; RNA Precursors - metabolism; RNA Processing, Post-Transcriptional; Saccharomyces cerevisiae; Saccharomyces cerevisiae - chemistry; Saccharomyces cerevisiae - metabolism; Saccharomyces cerevisiae Proteins - chemistry; Saccharomyces cerevisiae Proteins - metabolism; Studies; Yeast
• ISSN: 1932-6203; 1932-6203
• DOI: 10.1371/journal.pone.0068412

During the assembly process of ribosomal subunits, their structural components, the ribosomal RNAs (rRNAs) and the ribosomal proteins (r-proteins) have to join together in a highly dynamic and defined manner to enable the efficient formation of functional ribosomes. In this work, the assembly of large ribosomal subunit (LSU) r-proteins from the eukaryote S. cerevisiae was systematically investigated. Groups of LSU r-proteins with specific assembly characteristics were detected by comparing the protein composition of affinity purified early, middle, late or mature LSU (precursor) particles by semi-quantitative mass spectrometry. The impact of yeast LSU r-proteins rpL25, rpL2, rpL43, and rpL21 on the composition of intermediate to late nuclear LSU precursors was analyzed in more detail. Effects of these proteins on the assembly states of other r-proteins and on the transient LSU precursor association of several ribosome biogenesis factors, including Nog2, Rsa4 and Nop53, are discussed.