Live imaging of whole mouse embryos during gastrulation: migration analyses of epiblast and mesodermal cells

During gastrulation in the mouse embryo, dynamic cell movements including epiblast invagination and mesodermal layer expansion lead to the establishment of the three-layered body plan. The precise details of these movements, however, are sometimes elusive, because of the limitations in live imaging....

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Bibliographic Details
Published in:PloS one 2013-07, Vol.8 (7), p.e64506-e64506
Main Authors: Ichikawa, Takehiko, Nakazato, Kenichi, Keller, Philipp J, Kajiura-Kobayashi, Hiroko, Stelzer, Ernst H K, Mochizuki, Atsushi, Nonaka, Shigenori
Format: Article
Language:English
Subjects:
Animals
Biology
Cell cycle
Cell migration
Cell Movement
Cell Nucleus - metabolism
Cell Tracking
Embryo Culture Techniques
Embryo, Mammalian - cytology
Embryonic development
Embryos
Gastrulation
House mouse
Imaging, Three-Dimensional
Laboratories
Mesoderm - cytology
Mesoderm - metabolism
Mice
Microscopy
Neurosciences
Nuclei
Nuclei (cytology)
Science
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Summary:During gastrulation in the mouse embryo, dynamic cell movements including epiblast invagination and mesodermal layer expansion lead to the establishment of the three-layered body plan. The precise details of these movements, however, are sometimes elusive, because of the limitations in live imaging. To overcome this problem, we developed techniques to enable observation of living mouse embryos with digital scanned light sheet microscope (DSLM). The achieved deep and high time-resolution images of GFP-expressing nuclei and following 3D tracking analysis revealed the following findings: (i) Interkinetic nuclear migration (INM) occurs in the epiblast at embryonic day (E)6 and 6.5. (ii) INM-like migration occurs in the E5.5 embryo, when the epiblast is a monolayer and not yet pseudostratified. (iii) Primary driving force for INM at E6.5 is not pressure from neighboring nuclei. (iv) Mesodermal cells migrate not as a sheet but as individual cells without coordination.
ISSN:1932-6203
1932-6203
DOI:10.1371/journal.pone.0064506