Generation of mouse small intestinal epithelial cell lines that allow the analysis of specific innate immune functions

Cell lines derived from the small intestine that reflect authentic properties of the originating intestinal epithelium are of high value for studies on mucosal immunology and host microbial homeostasis. A novel immortalization procedure was applied to generate continuously proliferating cell lines f...

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Published in:PloS one 2013-08, Vol.8 (8), p.e72700-e72700
Main Authors: Schwerk, Johannes, Köster, Mario, Hauser, Hansjörg, Rohde, Manfred, Fulde, Marcus, Hornef, Mathias W, May, Tobias
Format: Article
Language:English
Subjects:
Animals
Antigens
Bacteria - immunology
Bacteria - pathogenicity
Biological response modifiers
Biomarkers - metabolism
Biotechnology
Cell culture
Cell Line
Cell lines
Cell Polarity - physiology
Cell Proliferation
Cytokines
Drug development
Embryo, Mammalian
Embryos
Epidemiology
Epithelial cells
Epithelium
Genes
Genetic engineering
Homeostasis
Immortalization
Immunity, Innate - physiology
Immunology
Infections
Inflammatory bowel disease
Interferon
Interferon Type I - metabolism
Intestinal Mucosa - cytology
Intestinal Mucosa - embryology
Intestinal Mucosa - immunology
Intestine, Small - cytology
Intestine, Small - embryology
Intestine, Small - immunology
Mice
Microorganisms
Microscopy
Mucosa
Primary Cell Culture - methods
Reproducibility
Rodents
Small intestine
Transgenic animals
Transgenic mice
Viruses
Viruses - immunology
Viruses - pathogenicity
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cited_by cdi_FETCH-LOGICAL-c692t-e829bd1f7e0e78e01f93a97e73fa3ebb00812d73356df61a75cd6f1bb6ed45753
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container_title PloS one
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creator Schwerk, Johannes
Köster, Mario
Hauser, Hansjörg
Rohde, Manfred
Fulde, Marcus
Hornef, Mathias W
May, Tobias
description Cell lines derived from the small intestine that reflect authentic properties of the originating intestinal epithelium are of high value for studies on mucosal immunology and host microbial homeostasis. A novel immortalization procedure was applied to generate continuously proliferating cell lines from murine E19 embryonic small intestinal tissue. The obtained cell lines form a tight and polarized epithelial cell layer, display characteristic tight junction, microvilli and surface protein expression and generate increasing transepithelial electrical resistance during in vitro culture. Significant up-regulation of Cxcl2 and Cxcl5 chemokine expression upon exposure to defined microbial innate immune stimuli and endogenous cytokines is observed. Cell lines were also generated from a transgenic interferon reporter (Mx2-Luciferase) mouse, allowing reporter technology-based quantification of the cellular response to type I and III interferon. Thus, the newly created cell lines mimic properties of the natural epithelium and can be used for diverse studies including testing of the absorption of drug candidates. The reproducibility of the method to create such cell lines from wild type and transgenic mice provides a new tool to study molecular and cellular processes of the epithelial barrier.
doi_str_mv 10.1371/journal.pone.0072700
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subjects Animals
Antigens
Bacteria - immunology
Bacteria - pathogenicity
Biological response modifiers
Biomarkers - metabolism
Biotechnology
Cell culture
Cell Line
Cell lines
Cell Polarity - physiology
Cell Proliferation
Cytokines
Drug development
Embryo, Mammalian
Embryos
Epidemiology
Epithelial cells
Epithelium
Genes
Genetic engineering
Homeostasis
Immortalization
Immunity, Innate - physiology
Immunology
Infections
Inflammatory bowel disease
Interferon
Interferon Type I - metabolism
Intestinal Mucosa - cytology
Intestinal Mucosa - embryology
Intestinal Mucosa - immunology
Intestine, Small - cytology
Intestine, Small - embryology
Intestine, Small - immunology
Mice
Microorganisms
Microscopy
Mucosa
Primary Cell Culture - methods
Reproducibility
Rodents
Small intestine
Transgenic animals
Transgenic mice
Viruses
Viruses - immunology
Viruses - pathogenicity
title Generation of mouse small intestinal epithelial cell lines that allow the analysis of specific innate immune functions
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