Linking plant nutritional status to plant-microbe interactions

• Published in: PloS one 2013-07, Vol.8 (7), p.e68555
• Main Authors: Carvalhais, Lilia C, Dennis, Paul G, Fan, Ben, Fedoseyenko, Dmitri, Kierul, Kinga, Becker, Anke, von Wiren, Nicolaus, Borriss, Rainer
• Format: Article
• Language: English
• Subjects: Adaptation; Agriculture; Amino acids; Bacillus amyloliquefaciens; Bacillus subtilis; Bacteria; Biology; Biosynthesis; Chemotaxis; Corn; Exudates; Exudation; Gene expression; Gene Expression Regulation, Plant; Genes; Hostages; Iron; Iron - metabolism; Iron and steel plants; Metabolomics; Microbiology; Microorganisms; Nitrogen; Nitrogen - metabolism; Nutrient concentrations; Nutrient deficiency; Nutrients; Nutrition; Nutritional status; Phase transitions; Phosphates; Phosphorus - metabolism; Plant genetics; Plant growth; Plant nutrition; Plant Roots - genetics; Plant Roots - metabolism; Plant Roots - microbiology; Potassium; Potassium - metabolism; Productivity; Protein biosynthesis; Protein synthesis; Rhizobiaceae - physiology; Seeds; Substrates; Transcription; Valine; Zea mays - genetics; Zea mays - metabolism; Zea mays - microbiology
• ISSN: 1932-6203; 1932-6203
• DOI: 10.1371/journal.pone.0068555

Plants have developed a wide-range of adaptations to overcome nutrient limitation, including changes to the quantity and composition of carbon-containing compounds released by roots. Root-associated bacteria are largely influenced by these compounds which can be perceived as signals or substrates. Here, we evaluate the effect of root exudates collected from maize plants grown under nitrogen (N), phosphate (P), iron (Fe) and potassium (K) deficiencies on the transcriptome of the plant growth promoting rhizobacterium (PGPR) Bacillus amyloliquefaciens FZB42. The largest shifts in gene expression patterns were observed in cells exposed to exudates from N-, followed by P-deficient plants. Exudates from N-deprived maize triggered a general stress response in FZB42 in the exponential growth phase, which was evidenced by the suppression of numerous genes involved in protein synthesis. Exudates from P-deficient plants induced bacterial genes involved in chemotaxis and motility whilst exudates released by Fe and K deficient plants did not cause dramatic changes in the bacterial transcriptome during exponential growth phase. Global transcriptional changes in bacteria elicited by nutrient deficient maize exudates were significantly correlated with concentrations of the amino acids aspartate, valine and glutamate in root exudates suggesting that transcriptional profiling of FZB42 associated with metabolomics of N, P, Fe and K-deficient maize root exudates is a powerful approach to better understand plant-microbe interactions under conditions of nutritional stress.