Aberrant expression of Beclin-1 and LC3 correlates with poor prognosis of human hypopharyngeal squamous cell carcinoma

Beclin-1, a key regulator of autophagy. Microtubule-associated protein 1 light chain 3 (LC3), is involved in autophagsome formation during autophagy. The autophagic genes beclin-1 and LC3 paly an important role in the development and progression of tumor. This study was designed to investigate the e...

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Published in:PloS one 2013-07, Vol.8 (7), p.e69038-e69038
Main Authors: Wang, Juan, Pan, Xin-Liang, Ding, Li-Jie, Liu, Da-Yu, Da-Peng Lei, Jin, Tong
Format: Article
Language:English
Subjects:
Aberration
Aged
Apoptosis
Apoptosis Regulatory Proteins - genetics
Autophagy
Beclin-1
Brain cancer
Breast cancer
Cancer therapies
Carcinoma, Squamous Cell - diagnosis
Carcinoma, Squamous Cell - genetics
Carcinoma, Squamous Cell - mortality
Cell death
Chemotherapy
Correlation
Ethics
Female
Gene Expression
Genes
Hospitals
Humans
Hypopharyngeal Neoplasms - diagnosis
Hypopharyngeal Neoplasms - genetics
Hypopharyngeal Neoplasms - mortality
Hypopharynx - metabolism
Hypopharynx - pathology
Immunohistochemistry
Liver cancer
Lymph nodes
Lymphatic Metastasis
Lymphoma
Lymphomas
Male
Medical prognosis
Melanoma
Membrane Proteins - genetics
Metastases
Microtubule-associated protein 1
Microtubule-Associated Proteins - genetics
Middle Aged
Molecular weight
Mucosa
Multivariate Analysis
Otolaryngology
Patients
Phagocytosis
Polymerase chain reaction
Prognosis
Proteins
Radiation therapy
RNA
Squamous cell carcinoma
Surgery
Survival
Survival Analysis
Throat cancer
Tissues
Transcription
Tumorigenesis
Tumors
Western blotting
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Summary:Beclin-1, a key regulator of autophagy. Microtubule-associated protein 1 light chain 3 (LC3), is involved in autophagsome formation during autophagy. The autophagic genes beclin-1 and LC3 paly an important role in the development and progression of tumor. This study was designed to investigate the expression of beclin-1 and LC3 and to clarify their clinical significance in hypopharyngeal squamous cell carcinoma (HSCC). Eighty-two surgical hypopharyngeal squamous cell carcinoma specimens and fifty-four adjacent non-cancerous mucosal epithelial tissues were obtained. Beclin-1 and LC3-II expression was examined by immunohistochemistry, real-time RT-PCR and Western blotting assays. Correlations with patient clinical characteristics and overall survival were evaluated. Beclin-1 was positively expressed in 42.7% (35/82) of HSCC specimens (adjacent non-cancerous tissues, 79.6%, 43/54; P
ISSN:1932-6203
1932-6203
DOI:10.1371/journal.pone.0069038