Dynamics of Notch pathway expression during mouse testis post-natal development and along the spermatogenic cycle

The transcription and expression patterns of Notch pathway components (Notch 1-3, Delta1 and 4, Jagged1) and effectors (Hes1, Hes2, Hes5 and Nrarp) were evaluated (through RT-PCR and IHC) in the mouse testis at key moments of post-natal development, and along the adult spermatogenic cycle. Notch pat...

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Bibliographic Details
Published in:PloS one 2013-08, Vol.8 (8), p.e72767-e72767
Main Authors: Murta, Daniel, Batista, Marta, Silva, Elisabete, Trindade, Alexandre, Henrique, Domingos, Duarte, António, Lopes-da-Costa, Luís
Format: Article
Language:English
Subjects:
Animals
Drosophila
Effectors
Gene expression
Gene Expression Regulation - physiology
Genetic engineering
Infertility
Insects
Jagged1 protein
Leydig cells
Ligands
Male
Mammals
Medical research
Mice
Notch protein
Notch1 protein
Obstetrics
Polymerase chain reaction
Puberty
Receptors, Notch - genetics
Receptors, Notch - metabolism
Rodents
Signal Transduction - physiology
Signaling
Spermatogenesis
Spermatogenesis - physiology
Spermatogonia
Studies
Testis - cytology
Testis - growth & development
Testis - metabolism
Testosterone
Transcription
Transcription, Genetic - physiology
Veterinary medicine
Windows (intervals)
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Summary:The transcription and expression patterns of Notch pathway components (Notch 1-3, Delta1 and 4, Jagged1) and effectors (Hes1, Hes2, Hes5 and Nrarp) were evaluated (through RT-PCR and IHC) in the mouse testis at key moments of post-natal development, and along the adult spermatogenic cycle. Notch pathway components and effectors are transcribed in the testis and expressed in germ, Sertoli and Leydig cells, and each Notch component shows a specific cell-type and time-window expression pattern. This expression at key testis developmental events prompt for a role of Notch signaling in pre-pubertal spermatogonia quiescence, onset of spermatogenesis, and regulation of the spermatogenic cycle.
ISSN:1932-6203
1932-6203
DOI:10.1371/journal.pone.0072767