Region specific response of intervertebral disc cells to complex dynamic loading: an organ culture study using a dynamic torsion-compression bioreactor

• Published in: PloS one 2013-08, Vol.8 (8), p.e72489-e72489
• Main Authors: Chan, Samantha C W, Walser, Jochen, Käppeli, Patrick, Shamsollahi, Mohammad Javad, Ferguson, Stephen J, Gantenbein-Ritter, Benjamin
• Format: Article
• Language: English
• Subjects: Animals; Apoptosis; Axial compression; Back pain; Biology; Biomechanics; Bioreactors; Cattle; Cell culture; Cell morphology; Compression; Compressive Strength; Cytology; Degeneration; Dynamic loads; Explants; Extracellular Matrix - metabolism; Extracellular Matrix - pathology; Extracellular Matrix Proteins - biosynthesis; Gene expression; Gene regulation; Genes; Glycosaminoglycans; Intervertebral Disc - metabolism; Intervertebral Disc - pathology; Intervertebral Disc Degeneration - metabolism; Intervertebral Disc Degeneration - pathology; Intervertebral discs; Mechanical loading; Mechanical properties; Medicine; Nuclei; Nucleus pulposus; Organ culture; Organ Culture Techniques; Physiology; Pressure distribution; Spine; Statistical analysis; Studies; Up-Regulation; Weight-Bearing
• ISSN: 1932-6203; 1932-6203
• DOI: 10.1371/journal.pone.0072489

The spine is routinely subjected to repetitive complex loading consisting of axial compression, torsion, flexion and extension. Mechanical loading is one of the important causes of spinal diseases, including disc herniation and disc degeneration. It is known that static and dynamic compression can lead to progressive disc degeneration, but little is known about the mechanobiology of the disc subjected to combined dynamic compression and torsion. Therefore, the purpose of this study was to compare the mechanobiology of the intervertebral disc when subjected to combined dynamic compression and axial torsion or pure dynamic compression or axial torsion using organ culture. We applied four different loading modalities [1. control: no loading (NL), 2. cyclic compression (CC), 3. cyclic torsion (CT), and 4. combined cyclic compression and torsion (CCT)] on bovine caudal disc explants using our custom made dynamic loading bioreactor for disc organ culture. Loads were applied for 8 h/day and continued for 14 days, all at a physiological magnitude and frequency. Our results provided strong evidence that complex loading induced a stronger degree of disc degeneration compared to one degree of freedom loading. In the CCT group, less than 10% nucleus pulposus (NP) cells survived the 14 days of loading, while cell viabilities were maintained above 70% in the NP of all the other three groups and in the annulus fibrosus (AF) of all the groups. Gene expression analysis revealed a strong up-regulation in matrix genes and matrix remodeling genes in the AF of the CCT group. Cell apoptotic activity and glycosaminoglycan content were also quantified but there were no statistically significant differences found. Cell morphology in the NP of the CCT was changed, as shown by histological evaluation. Our results stress the importance of complex loading on the initiation and progression of disc degeneration.