Generating a non-integrating human induced pluripotent stem cell bank from urine-derived cells

Induced pluripotent stem cell (iPS cell) holds great potential for applications in regenerative medicine, drug discovery, and disease modeling. We describe here a practical method to generate human iPS cells from urine-derived cells (UCs) under feeder-free, virus-free, serum-free condition and witho...

Full description

Saved in:
Bibliographic Details
Published in:PloS one 2013-08, Vol.8 (8), p.e70573-e70573
Main Authors: Xue, Yanting, Cai, Xiujuan, Wang, Linli, Liao, Baojian, Zhang, Hui, Shan, Yongli, Chen, Qianyu, Zhou, Tiancheng, Li, Xirui, Hou, Jundi, Chen, Shubin, Luo, Rongping, Qin, Dajiang, Pei, Duanqing, Pan, Guangjin
Format: Article
Language:English
Subjects:
Adolescent
Adult
Airlines
Amyotrophic lateral sclerosis
Banks (Finance)
Biology
Biotechnology
c-Myc protein
Cell Culture Techniques - methods
Cell lines
Chief executive officers
Child
Child, Preschool
Embryos
Female
Genetic diversity
Humans
Induced Pluripotent Stem Cells - cytology
Male
Medicine
Middle Aged
Myc protein
Pluripotency
Population genetics
Purchase orders
Regenerative medicine
Stem cells
Urine
Urine - cytology
Viruses
Young Adult
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Induced pluripotent stem cell (iPS cell) holds great potential for applications in regenerative medicine, drug discovery, and disease modeling. We describe here a practical method to generate human iPS cells from urine-derived cells (UCs) under feeder-free, virus-free, serum-free condition and without oncogene c-MYC. We showed that this approach could be applied in a large population with different genetic backgrounds. UCs are easily accessible and exhibit high reprogramming efficiency, offering advantages over other cell types used for the purpose of iPS generation. Using the approach described in this study, we have generated 93 iPS cell lines from 20 donors with diverse genetic backgrounds. The non-viral iPS cell bank with these cell lines provides a valuable resource for iPS cells research, facilitating future applications of human iPS cells.
ISSN:1932-6203
1932-6203
DOI:10.1371/journal.pone.0070573