Expansion of multipotent stem cells from the adult human brain

The discovery of stem cells in the adult human brain has revealed new possible scenarios for treatment of the sick or injured brain. Both clinical use of and preclinical research on human adult neural stem cells have, however, been seriously hampered by the fact that it has been impossible to passag...

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Bibliographic Details
Published in:PloS one 2013-08, Vol.8 (8), p.e71334-e71334
Main Authors: Murrell, Wayne, Palmero, Emily, Bianco, John, Stangeland, Biljana, Joel, Mrinal, Paulson, Linda, Thiede, Bernd, Grieg, Zanina, Ramsnes, Ingunn, Skjellegrind, Håvard K, Nygård, Ståle, Brandal, Petter, Sandberg, Cecilie, Vik-Mo, Einar, Palmero, Sheryl, Langmoen, Iver A
Format: Article
Language:English
Subjects:
Adult
Biology
Biomarkers - metabolism
Brain
Brain - cytology
Brain research
Brain stem
Cell Culture Techniques - methods
Cell Differentiation
Chromosomes
Clone Cells - cytology
Dopaminergic Neurons - cytology
Expansion
Extracellular matrix
Gene expression
Gene Expression Regulation
Genotype & phenotype
Hospitals
Humans
In vitro fertilization
Karyotyping
Laboratories
Medical research
Medicine
Middle Aged
Multipotent Stem Cells - cytology
Multipotent Stem Cells - metabolism
Nervous system
Neural stem cells
Oct-4 protein
Phenotype
Pluripotency
Proteins
Proteomics
Staining and Labeling
Stem cell research
Stem cell transplantation
Stem cells
Tissues
Transplantation
Transplants & implants
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Summary:The discovery of stem cells in the adult human brain has revealed new possible scenarios for treatment of the sick or injured brain. Both clinical use of and preclinical research on human adult neural stem cells have, however, been seriously hampered by the fact that it has been impossible to passage these cells more than a very few times and with little expansion of cell numbers. Having explored a number of alternative culturing conditions we here present an efficient method for the establishment and propagation of human brain stem cells from whatever brain tissue samples we have tried. We describe virtually unlimited expansion of an authentic stem cell phenotype. Pluripotency proteins Sox2 and Oct4 are expressed without artificial induction. For the first time multipotency of adult human brain-derived stem cells is demonstrated beyond tissue boundaries. We characterize these cells in detail in vitro including microarray and proteomic approaches. Whilst clarification of these cells' behavior is ongoing, results so far portend well for the future repair of tissues by transplantation of an adult patient's own-derived stem cells.
ISSN:1932-6203
1932-6203
DOI:10.1371/journal.pone.0071334