A two-component system regulates hemin acquisition in Porphyromonas gingivalis

Porphyromonas gingivalis is a Gram-negative oral anaerobe associated with infection of the periodontia. The organism has a small number of two-component signal transduction systems, and after comparing genome sequences of strains W83 and ATCC 33277 we discovered that the latter was mutant in histidi...

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Bibliographic Details
Published in:PloS one 2013-09, Vol.8 (9), p.e73351
Main Authors: Scott, Jodie C, Klein, Brian A, Duran-Pinedo, Ana, Hu, Linden, Duncan, Margaret J
Format: Article
Language:English
Subjects:
Amino Acid Sequence
Bacterial Proteins - chemistry
Bacterial Proteins - genetics
Bacterial Proteins - metabolism
Bacteroidaceae Infections - metabolism
Bacteroidaceae Infections - microbiology
Cellular signal transduction
Cloning
DNA microarrays
E coli
Electrophoretic mobility
Gene expression
Gene Expression Regulation, Bacterial
Gene sequencing
Genes, Bacterial
Genomes
Hemin
Hemin - metabolism
Hemoglobin
Histidine
Histidine Kinase
Host-Pathogen Interactions
Humans
Iron - metabolism
Kinases
Molecular Sequence Data
Mutation
Periodontics
Plasmids
Porphyromonas gingivalis
Porphyromonas gingivalis - genetics
Porphyromonas gingivalis - physiology
Protein Kinases - chemistry
Protein Kinases - genetics
Protein Kinases - metabolism
Proteins
Signal Transduction
Transcription
Transduction
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Summary:Porphyromonas gingivalis is a Gram-negative oral anaerobe associated with infection of the periodontia. The organism has a small number of two-component signal transduction systems, and after comparing genome sequences of strains W83 and ATCC 33277 we discovered that the latter was mutant in histidine kinase (PGN_0752), while the cognate response regulator (PGN_0753) remained intact. Microarray-based transcriptional profiling and ChIP-seq assays were carried out with an ATCC 33277 transconjugant containing the functional histidine kinase from strain W83 (PG0719). The data showed that the regulon of this signal transduction system contained genes that were involved in hemin acquisition, including gingipains, at least three transport systems, as well as being self-regulated. Direct regulation by the response regulator was confirmed by electrophoretic mobility shift assays. In addition, the system appears to be activated by hemin and the regulator acts as both an activator and repressor.
ISSN:1932-6203
1932-6203
DOI:10.1371/journal.pone.0073351