xopAC-triggered immunity against Xanthomonas depends on Arabidopsis receptor-like cytoplasmic kinase genes PBL2 and RIPK

• Published in: PloS one 2013-08, Vol.8 (8), p.e73469-e73469
• Main Authors: Guy, Endrick, Lautier, Martine, Chabannes, Matthieu, Roux, Brice, Lauber, Emmanuelle, Arlat, Matthieu, Noël, Laurent D
• Format: Article
• Language: English
• Subjects: Arabidopsis; Arabidopsis - genetics; Arabidopsis - immunology; Arabidopsis - microbiology; Arabidopsis Proteins - genetics; Arabidopsis Proteins - metabolism; Arabidopsis thaliana; Bacteria; Bacterial Proteins - genetics; Black rot; Cell Membrane - enzymology; Cell Membrane - genetics; Cloning; Cytoplasm - enzymology; Cytoplasm - genetics; Disease; Gene Expression Regulation, Plant; Genes; Genes, Reporter; Genetic research; Gram-negative bacteria; Host-Pathogen Interactions; Immunity; Kinases; Life Sciences; Luminescent Proteins - genetics; Medical screening; Mesophyll; Pathogens; Phosphorylation; Phosphotransferases; Plant Cells - metabolism; Plant Cells - microbiology; Plant Diseases - genetics; Plant Diseases - immunology; Plant Diseases - microbiology; Plant Immunity - genetics; Plant resistance; Plasmids; Protein Kinases - genetics; Protein Kinases - metabolism; Protein-Serine-Threonine Kinases - genetics; Protein-Serine-Threonine Kinases - metabolism; Proteins; Pseudomonas syringae; Pseudomonas syringae - physiology; Ralstonia solanacearum; Ralstonia solanacearum - physiology; Regulators; Signal transduction; Uridylyl transferase; Vegetal Biology; Xanthomonas campestris; Xanthomonas campestris - physiology; Yeast
• ISSN: 1932-6203; 1932-6203
• DOI: 10.1371/journal.pone.0073469

Xanthomonas campestris pv. campestris (Xcc) colonizes the vascular system of Brassicaceae and ultimately causes black rot. In susceptible Arabidopsis plants, XopAC type III effector inhibits by uridylylation positive regulators of the PAMP-triggered immunity such as the receptor-like cytoplasmic kinases (RLCK) BIK1 and PBL1. In the resistant ecotype Col-0, xopAC is a major avirulence gene of Xcc. In this study, we show that both the RLCK interaction domain and the uridylyl transferase domain of XopAC are required for avirulence. Furthermore, xopAC can also confer avirulence to both the vascular pathogen Ralstonia solanacearum and the mesophyll-colonizing pathogen Pseudomonas syringae indicating that xopAC-specified effector-triggered immunity is not specific to the vascular system. In planta, XopAC-YFP fusions are localized at the plasma membrane suggesting that XopAC might interact with membrane-localized proteins. Eight RLCK of subfamily VII predicted to be localized at the plasma membrane and interacting with XopAC in yeast two-hybrid assays have been isolated. Within this subfamily, PBL2 and RIPK RLCK genes but not BIK1 are important for xopAC-specified effector-triggered immunity and Arabidopsis resistance to Xcc.