Targeted mutagenesis in the malaria mosquito using TALE nucleases

Anopheles gambiae, the main mosquito vector of human malaria, is a challenging organism to manipulate genetically. As a consequence, reverse genetics studies in this disease vector have been largely limited to RNA interference experiments. Here, we report the targeted disruption of the immunity gene...

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Bibliographic Details
Published in:PloS one 2013-08, Vol.8 (8), p.e74511
Main Authors: Smidler, Andrea L, Terenzi, Olivier, Soichot, Julien, Levashina, Elena A, Marois, Eric
Format: Article
Language:English
Subjects:
Amino Acid Sequence
Amino acids
Animals
Anopheles
Anopheles - genetics
Anopheles - parasitology
Anopheles gambiae
Aquatic insects
Base Sequence
Biotechnology
Culicidae
Deoxyribonucleic acid
Disease control
DNA
DNA binding proteins
Endonucleases - metabolism
Gene expression
Gene Targeting
Genes
Genes, Insect
Genetic analysis
Genetic aspects
Genetic engineering
Genetics
Genomes
Humans
Immunity
Insect Proteins - chemistry
Insect Proteins - genetics
Insecticides
Laboratories
Malaria
Malaria - parasitology
Molecular Sequence Data
Mosquitoes
Mutagenesis
Mutagenesis - genetics
Mutant Proteins - chemistry
Mutant Proteins - genetics
Mutants
Mutation
Mutation - genetics
Nuclease
Nucleases
Parasites
Plasmodium
Plasmodium berghei - physiology
Plasmodium falciparum
Proteins
Ribonucleic acid
RNA
RNA-mediated interference
Site-directed mutagenesis
Targeted mutagenesis
Trans-Activators - metabolism
Transcription
Transcription (Genetics)
Transcription activator-like effector nucleases
Vector-borne diseases
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Description
Summary:Anopheles gambiae, the main mosquito vector of human malaria, is a challenging organism to manipulate genetically. As a consequence, reverse genetics studies in this disease vector have been largely limited to RNA interference experiments. Here, we report the targeted disruption of the immunity gene TEP1 using transgenic expression of Transcription-Activator Like Effector Nucleases (TALENs), and the isolation of several TEP1 mutant A. gambiae lines. These mutations inhibited protein production and rendered TEP1 mutants hypersusceptible to Plasmodium berghei. The TALEN technology opens up new avenues for genetic analysis in this disease vector and may offer novel biotechnology-based approaches for malaria control.
ISSN:1932-6203
1932-6203
DOI:10.1371/journal.pone.0074511