The TRPA1 agonist, methyl syringate suppresses food intake and gastric emptying

• Published in: PloS one 2013-08, Vol.8 (8), p.e71603-e71603
• Main Authors: Kim, Min Jung, Son, Hee Jin, Song, Seo Hyeon, Jung, Myungji, Kim, Yiseul, Rhyu, Mee-Ra
• Format: Article
• Language: English
• Subjects: Acetanilides - chemistry; Acrolein - analogs & derivatives; Acrolein - pharmacology; Animals; Biology; Coloring Agents - pharmacology; Diabetes; Eating - drug effects; Emptying; Feeding Behavior - drug effects; Food; Food intake; Gallic Acid - analogs & derivatives; Gallic Acid - pharmacology; Gastric emptying; Gastric Emptying - drug effects; Gastric motility; Gastroenterology; Gastrointestinal Hormones - pharmacology; Gastrointestinal system; Gastrointestinal tract; Gene expression; Glucagon; Glucagon-like peptide 1; Glucagon-Like Peptide 1 - metabolism; Hormones; Immunoassay; Imprinting; Insulin; Laboratory animals; Male; Mathematics; Medicine; Metabolism; Mice; Mice, Inbred ICR; Models, Chemical; Peptide YY - metabolism; Peptides; Phenols; Platinum group compounds; Polypeptides; Protein Structure, Tertiary; Proteins; Purines - chemistry; Rodents; Ruthenium; Ruthenium red; Ruthenium Red - chemistry; Ruthenium Red - pharmacology; Studies; Syringate; Transient Receptor Potential Channels - agonists; Transient receptor potential proteins; TRPA1 Cation Channel
• ISSN: 1932-6203; 1932-6203
• DOI: 10.1371/journal.pone.0071603

Transient receptor potential channel ankryn 1 (TRPA1) expressed in the gastrointestinal tract is associated with gastric motility, gastric emptying, and food intake. In this study, we investigated the effects of methyl syringate, a specific and selective TRPA1 agonist, on food intake, gastric emptying, and gut hormone levels in imprinting control region (ICR) mice. The administration of methyl syringate suppressed cumulative food intake and gastric emptying. In addition, treatment with ruthenium red (RR), a general cation channel blocker, and HC-030031, a selective TRPA1 antagonist, inhibited methyl syringate-induced reduction of food intake and delayed gastric emptying in ICR mice. Methyl syringate also increased plasma peptide YY (PYY) levels, but not glucagon-like peptide-1 (GLP-1) levels. The elevation in PYY was blocked by treatment with RR and HC-030031. The present findings indicate that methyl syringate regulates food intake and gastric emptying through a TRPA1-mediated pathway and, by extension, can contribute to weight suppression.