Growth prior to thermogenesis for a quick fledging of Adélie penguin chicks (Pygoscelis adeliae)

• Published in: PloS one 2013-09, Vol.8 (9), p.e74154-e74154
• Main Authors: Dégletagne, Cyril, Roussel, Damien, Rouanet, Jean Louis, Baudimont, Fanny, Moureaux, Elodie-Marie, Harvey, Steve, Duchamp, Claude, Le Maho, Yvon, Raccurt, Mireille
• Format: Article
• Language: English
• Subjects: Animals; Animals, Newborn; Antarctic Regions; Birds; Body Temperature Regulation - genetics; Breeding; Breeding seasons; Brittleness; Cell activation; Chicks; Cold; Cold Temperature; Cold water; Environmental Sciences; Fatty acids; Gene expression; Gene Expression Profiling; Gene Expression Regulation, Developmental; Gene regulation; Genes; Hatching; Insulin-like growth factor I; Insulin-like growth factors; Juveniles; Life Sciences; Lipid metabolism; Maturation; Metabolic Networks and Pathways - genetics; Metabolism; Muscles; Non-shivering; Oxidative metabolism; Paracrine Communication - genetics; Paracrine signalling; Pectoralis Muscles - growth & development; Pectoralis Muscles - metabolism; Physiology; Pygoscelis adeliae; Rodents; Shivering; Skeletal muscle; Spheniscidae - genetics; Spheniscidae - growth & development; Spheniscidae - metabolism; Substrates; Thermogenesis; Thermoregulation; Transcription; Transcription activation; Water circulation
• ISSN: 1932-6203; 1932-6203
• DOI: 10.1371/journal.pone.0074154

The evolutionary trade-off between tissue growth and mature function restricts the post natal development of polar birds. The present study uses an original integrative approach as it includes gene expression, plus biochemical and physiological analysis to investigate how Adélie penguin chicks achieve a rapid growth despite the energetic constraints linked to the cold and the very short breeding season in Antarctica. In pectoralis muscle, the main thermogenic tissue in birds, our data show that the transition from ectothermy to endothermy on Day 15 post- hatching is associated with substantial and coordinated changes in the transcription of key genes. While the early activation of genes controlling cell growth and differentiation (avGHR, avIGF-1R, T3Rβ) is rapidly down-regulated after hatching, the global increase in the relative expression of genes involved in thermoregulation (avUCP, avANT, avLPL) and transcriptional regulation (avPGC1α, avT3Rβ) underlie the muscular acquisition of oxidative metabolism. Adélie chicks only become real endotherms at 15 days of age with the development of an oxidative muscle phenotype and the ability to shiver efficiently. The persistent muscular expression of IGF-1 throughout growth probably acts as a local mediator to adjust muscle size and its oxidative capacity to anticipate the new physiological demands of future Dives in cold water. The up-regulation of T3Rβ mRNA levels suggests that circulating T3 may play an important role in the late maturation of skeletal muscle by reinforcing, at least in part, the paracrine action of IGF-1. From day 30, the metabolic shift from mixed substrate to lipid metabolism, with the markedly increased mRNA levels of muscle avLPL, avANT and avUCP, suggests the late development of a fatty acid-enhanced muscle non-shivering thermogenesis mechanism. This molecular control is the key to this finely-tuned strategy by which the Adélie penguin chick successfully heads for the sea on schedule.