Potentially functional polymorphisms in the CASP7 gene contribute to gastric adenocarcinoma susceptibility in an eastern Chinese population

• Published in: PloS one 2013-09, Vol.8 (9), p.e74041-e74041
• Main Authors: Wang, Meng-Yun, Zhu, Mei-Ling, He, Jing, Shi, Ting-Yan, Li, Qiao-Xin, Wang, Ya-Nong, Li, Jin, Zhou, Xiao-Yan, Sun, Meng-Hong, Wang, Xiao-Feng, Yang, Ya-Jun, Wang, Jiu-Cun, Jin, Li, Wei, Qing-Yi
• Format: Article
• Language: English
• Subjects: 3' Untranslated regions; Adenocarcinoma; Adenocarcinoma - genetics; Adult; Aged; Aged, 80 and over; Alleles; Analysis; Apoptosis; Asian Continental Ancestry Group - genetics; Binding sites; Cancer; Cancer genetics; Cancer research; Case-Control Studies; Caspase; Caspase 7 - genetics; Caspase-7; Cell Line, Transformed; China; Chromosomes; Confidence intervals; Control methods; Development and progression; Disease susceptibility; Education; Epidemiology; Female; Females; Gastric cancer; Gene expression; Gene Frequency; Gene Order; Genetic aspects; Genetic engineering; Genetic Predisposition to Disease; Genomes; Genotype; Genotypes; Health risks; Health sciences; Humans; Laboratories; Life sciences; Linkage Disequilibrium; Lung cancer; Male; MicroRNA; MicroRNAs; Middle Aged; miRNA; Mutation; Oncology; Patients; Polymorphism, Single Nucleotide; Population; Prognosis; Regression analysis; Ribonucleic acid; Risk analysis; Risk factors; RNA; RNA, Messenger - genetics; Single nucleotide polymorphisms; Single-nucleotide polymorphism; Smoking; Statistical analysis; Stomach cancer; Stomach Neoplasms - genetics; Studies; Subgroups; Young Adult
• ISSN: 1932-6203; 1932-6203
• DOI: 10.1371/journal.pone.0074041

Caspase 7 (CASP7) is an important regulator and executioner in the apoptosis pathway and plays a crucial role in cancer development and progression. However, few studies have evaluated associations between functional single nucleotide polymorphisms (SNPs) in the 3' untranslational region (UTR) of CASP7 and risk of gastric cancer. In a case-control study of 1117 patients with gastric cancer and 1146 cancer-free controls with frequency matching on age and sex, we genotyped four potentially functional SNPs (rs4353229T>C, rs10787498T>G, rs1127687G>A and rs12247479G>A) located in the microRNA binding sites of the CASP7 3' UTR by using Taqman assays and evaluated their associations with risk of gastric cancer by using logistic regression analyses as well as multifactorial dimension reduction (MDR) analysis. In the single-locus analysis, only the CASP7 rs4353229 TT genotype was associated with 0.83-fold decreased risk (95% confidence interval [CI] = 0.70-0.98) of gastric cancer under a recessive model, compared with the CT/CC genotypes. In the combined analysis of all four SNPs, we found that the risk of gastric cancer decreased by 19% in those carrying any of the risk genotypes (adjusted odds ratio = 0.81, 95% CI = 0.68-0.96), compared with those carrying zero risk genotypes, and this risk was more evident in subgroups of younger age (