Functional characterization of a juvenile hormone esterase related gene in the moth Sesamia nonagrioides through RNA interference

Juvenile hormone esterase (JHE) is a carboxylesterase that has attracted great interest because of its critical role in regulating larval to adult transition in insects and other arthropods. Previously, we characterized an ecdysteroid sensitive and juvenile hormone non-susceptible juvenile hormone e...

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Bibliographic Details
Published in:PloS one 2013-09, Vol.8 (9), p.e73834
Main Authors: Kontogiannatos, Dimitrios, Swevers, Luc, Maenaka, Katsumi, Park, Enoch Y, Iatrou, Kostas, Kourti, Anna
Format: Article
Language:English
Subjects:
Agricultural biotechnology
Animals
Arthropods
Bacteria
Baculoviridae - genetics
Baculovirus
Biosynthesis
Butterflies & moths
Carboxylesterase
Carboxylic Ester Hydrolases - genetics
Carboxylic Ester Hydrolases - metabolism
Corn
Double-stranded RNA
Gene expression
Genes
Genetic Vectors - genetics
Genetics
Genomes
Genomics
Green Fluorescent Proteins - genetics
Green Fluorescent Proteins - metabolism
Hemolymph
Hemolymph - metabolism
Hormones
Hydrolases
Insect Proteins - genetics
Insect Proteins - metabolism
Insects
Interference
Juvenile hormone esterase
Laboratories
Larva - genetics
Larva - growth & development
Larva - metabolism
Larval development
Microscopy, Fluorescence
Molecular biology
Molting
Moths - genetics
Moths - growth & development
Reverse Genetics - methods
Reverse Transcriptase Polymerase Chain Reaction
Ribonucleic acid
RNA
RNA Interference
RNA, Messenger - genetics
RNA, Messenger - metabolism
RNA-mediated interference
Sesamia nonagrioides
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Summary:Juvenile hormone esterase (JHE) is a carboxylesterase that has attracted great interest because of its critical role in regulating larval to adult transition in insects and other arthropods. Previously, we characterized an ecdysteroid sensitive and juvenile hormone non-susceptible juvenile hormone esterase related gene (SnJHER) in the corn stalk borer, Sesamia nonagrioides. SnJHER was rhythmically up-regulated close to each molt during the corn stalk borer's larval development. In this paper we attempted to functionally characterize SnJHER using several reverse genetics techniques. To functionally characterize SnJHER, we experimented with different dsRNA administration methods, including hemolymph, bacterial or baculovirus-mediated RNA interference, (RNAi). Our findings indicate the potential implication of SnJHER in the developmental programming of Sesamia nonagrioides. It is still unclear whether SnJHER is closely related to the authentic JHE gene, with different or similar biological functions.
ISSN:1932-6203
1932-6203
DOI:10.1371/journal.pone.0073834