Contrasting quiescent G0 phase with mitotic cell cycling in the mouse immune system

A transgenic mouse line expressing Fucci (fluorescent ubiquitination-based cell-cycle indicator) probes allows us to monitor the cell cycle in the hematopoietic system. Two populations with high and low intensities of Fucci signals for Cdt1(30/120) accumulation were identified by FACS analysis, and...

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Bibliographic Details
Published in:PloS one 2013-09, Vol.8 (9), p.e73801-e73801
Main Authors: Tomura, Michio, Sakaue-Sawano, Asako, Mori, Yoshiko, Takase-Utsugi, Mitsuyo, Hata, Akihiro, Ohtawa, Kenji, Kanagawa, Osami, Miyawaki, Atsushi
Format: Article
Language:English
Subjects:
Animals
Antigens
Bone marrow
Brain research
Cell cycle
Cell Cycle - physiology
Cell Division - physiology
Cell growth
Cells, Cultured
Cycles
Experiments
Female
Flow Cytometry
Fluorescence
Fluorescent indicators
G1 Phase - physiology
Gene expression
Hematopoietic system
Hematopoietic System - cytology
Immune response
Immune system
Immune System - cytology
Immunoglobulins
Immunology
Laboratories
Male
Mice
Mice, Transgenic
Organs
Resting Phase, Cell Cycle - physiology
Rodents
Science
Transgenic animals
Transgenic mice
Ubiquitination
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Summary:A transgenic mouse line expressing Fucci (fluorescent ubiquitination-based cell-cycle indicator) probes allows us to monitor the cell cycle in the hematopoietic system. Two populations with high and low intensities of Fucci signals for Cdt1(30/120) accumulation were identified by FACS analysis, and these correspond to quiescent G0 and cycling G1 cells, respectively. We observed the transition of immune cells between quiescent and proliferative phases in lymphoid organs during differentiation and immune responses.
ISSN:1932-6203
1932-6203
DOI:10.1371/journal.pone.0073801