A study on the geophylogeny of clinical and environmental Vibrio cholerae in Kenya

Cholera remains a significant public health challenge in many sub-Saharan countries including Kenya. We have performed a combination of phylogenetic and phenotypic analysis based on whole genome DNA sequences derived from 40 environmental and 57 clinical V. cholerae from different regions of Kenya i...

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Bibliographic Details
Published in:PloS one 2013-09, Vol.8 (9), p.e74829
Main Authors: Kiiru, John, Mutreja, Ankur, Mohamed, Ahmed Abade, Kimani, Racheal W, Mwituria, Joyce, Sanaya, Robert Onsare, Muyodi, Jane, Revathi, Gunturu, Parkhill, Julian, Thomson, Nicholas, Dougan, Gordon, Kariuki, Samuel
Format: Article
Language:English
Subjects:
Antibiotic resistance
Antibiotics
Bioinformatics
Cholera
Cholera toxin
Clinical isolates
Deoxyribonucleic acid
DNA
DNA sequencing
Epidemics
Gene sequencing
Genomes
Genomics
Humans
Infections
Kenya
Medical research
Microbial drug resistance
Molecular Sequence Data
Nucleotide sequence
Pandemics
Pathogens
Phylogenetics
Phylogeny
Public health
Sediments
Vibrio cholerae
Vibrio cholerae - classification
Vibrio cholerae - genetics
Water-borne diseases
Waterborne diseases
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Summary:Cholera remains a significant public health challenge in many sub-Saharan countries including Kenya. We have performed a combination of phylogenetic and phenotypic analysis based on whole genome DNA sequences derived from 40 environmental and 57 clinical V. cholerae from different regions of Kenya isolated between 2005 and 2010. Some environmental and all clinical isolates mapped back onto wave three of the monophyletic seventh pandemic V. cholerae El Tor phylogeny but other environmental isolates were phylogenetically very distinct. Thus, the genomes of the Kenyan V. cholerae O1 El Tor isolates are clonally related to other El Tor V. cholerae isolated elsewhere in the world and similarly harbour antibiotic resistance-associated STX elements. Further, the Kenyan O1 El Tor isolates fall into two distinct clades that may have entered Kenya independently.
ISSN:1932-6203
1932-6203
DOI:10.1371/journal.pone.0074829