Impact of microRNA expression in human atrial tissue in patients with atrial fibrillation undergoing cardiac surgery

Although microRNA (miRNA) regulates initiation and/or progression of atrial fibrillation (AF) in canine AF models, the underlying mechanism in humans remains unclear. We speculated that certain miRNAs in atrial tissue are related to AF, and evaluated the relationship of miRNA expression in human atr...

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Published in:PloS one 2013-09, Vol.8 (9), p.e73397-e73397
Main Authors: Nishi, Hiroyuki, Sakaguchi, Taichi, Miyagawa, Shigeru, Yoshikawa, Yasushi, Fukushima, Satsuki, Saito, Shunsuke, Ueno, Takayoshi, Kuratani, Toru, Sawa, Yoshiki
Format: Article
Language:English
Subjects:
Aged
Atrial fibrillation
Atrial Fibrillation - genetics
Atrial Fibrillation - metabolism
Biomarkers
Cardiac arrhythmia
Cardiac Surgical Procedures
Cardiovascular disease
Care and treatment
Coronary vessels
DNA microarrays
DNA polymerases
Electrocardiography
Fibrillation
Fibrosis
Heart
Heart Atria - metabolism
Heart diseases
Heart failure
Heart surgery
Humans
Medicine
MicroRNA
MicroRNAs
MicroRNAs - genetics
Middle Aged
miRNA
Patients
Plasma levels
Polymerase chain reaction
Reverse Transcriptase Polymerase Chain Reaction
Ribonucleic acid
RNA
RNA-directed DNA polymerase
Sinus
Studies
Surgery
Tissues
University graduates
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creator Nishi, Hiroyuki
Sakaguchi, Taichi
Miyagawa, Shigeru
Yoshikawa, Yasushi
Fukushima, Satsuki
Saito, Shunsuke
Ueno, Takayoshi
Kuratani, Toru
Sawa, Yoshiki
description Although microRNA (miRNA) regulates initiation and/or progression of atrial fibrillation (AF) in canine AF models, the underlying mechanism in humans remains unclear. We speculated that certain miRNAs in atrial tissue are related to AF, and evaluated the relationship of miRNA expression in human atrial tissue in cardiac surgery patients. Right atrial tissues from 29 patients undergoing cardiovascular surgery were divided into 3 groups [A: chronic AF or unsuccessful maze, n=6; B: successful maze, n=10; C: sinus rhythm (SR) n=13]. miRNA expression was determined using high density microarrays and with Reverse transcriptase-polymerase chain reaction (RT-PCR). Fibrosis was examined using Masson trichrome staining. miRNA microarray analysis showed elevated miRNA-21, miRNA-23b, miRNA-199b, and miRNA-208b in AF as compared to SR groups. RT-PCR showed elevated miRNA-21 (1.9-fold) and miRNA-208b (4.2-fold) in AF as compared to the SR groups. miRNA-21 expression increased from Group C to A (A: 2.1-fold, B: 1.8-fold, C: 1.0-fold). Fibrosis increased from C to A (A: 43.0±12.9%, B: 21.3±6.1%, C: 11.9±3.1%). Percent fibrosis and miRNA-21 expression were correlated (r=0.508, p
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We speculated that certain miRNAs in atrial tissue are related to AF, and evaluated the relationship of miRNA expression in human atrial tissue in cardiac surgery patients. Right atrial tissues from 29 patients undergoing cardiovascular surgery were divided into 3 groups [A: chronic AF or unsuccessful maze, n=6; B: successful maze, n=10; C: sinus rhythm (SR) n=13]. miRNA expression was determined using high density microarrays and with Reverse transcriptase-polymerase chain reaction (RT-PCR). Fibrosis was examined using Masson trichrome staining. miRNA microarray analysis showed elevated miRNA-21, miRNA-23b, miRNA-199b, and miRNA-208b in AF as compared to SR groups. RT-PCR showed elevated miRNA-21 (1.9-fold) and miRNA-208b (4.2-fold) in AF as compared to the SR groups. miRNA-21 expression increased from Group C to A (A: 2.1-fold, B: 1.8-fold, C: 1.0-fold). Fibrosis increased from C to A (A: 43.0±12.9%, B: 21.3±6.1%, C: 11.9±3.1%). Percent fibrosis and miRNA-21 expression were correlated (r=0.508, p&lt;0.05). The plasma levels of miRNA-21 in AF patients was significantly decreased as compared to the healthy volunteers (p&lt;0.05). The expression of miRNA-21 in human atrial tissue was found to be related to atrial fibrosis and might affect AF occurrence, indicating its usefulness as a biomarker for cardiac surgery management.</abstract><cop>United States</cop><pub>Public Library of Science</pub><pmid>24069193</pmid><doi>10.1371/journal.pone.0073397</doi><tpages>e73397</tpages><oa>free_for_read</oa></addata></record>
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1932-6203
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subjects Aged
Atrial fibrillation
Atrial Fibrillation - genetics
Atrial Fibrillation - metabolism
Biomarkers
Cardiac arrhythmia
Cardiac Surgical Procedures
Cardiovascular disease
Care and treatment
Coronary vessels
DNA microarrays
DNA polymerases
Electrocardiography
Fibrillation
Fibrosis
Heart
Heart Atria - metabolism
Heart diseases
Heart failure
Heart surgery
Humans
Medicine
MicroRNA
MicroRNAs
MicroRNAs - genetics
Middle Aged
miRNA
Patients
Plasma levels
Polymerase chain reaction
Reverse Transcriptase Polymerase Chain Reaction
Ribonucleic acid
RNA
RNA-directed DNA polymerase
Sinus
Studies
Surgery
Tissues
University graduates
title Impact of microRNA expression in human atrial tissue in patients with atrial fibrillation undergoing cardiac surgery
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