Proteomic-based insight into Malpighian tubules of silkworm Bombyx mori

Malpighian tubules (MTs) are highly specific organs of arthropods (Insecta, Myriapoda and Arachnida) for excretion and osmoregulation. In order to highlight the important genes and pathways involved in multi-functions of MTs, we performed a systematic proteomic analysis of silkworm MTs in the presen...

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Bibliographic Details
Published in:PloS one 2013-09, Vol.8 (9), p.e75731-e75731
Main Authors: Zhong, Xiao-wu, Zou, Yong, Liu, Shi-ping, Yi, Qi-ying, Hu, Cui-mei, Wang, Chen, Xia, Qing-you, Zhao, Ping
Format: Article
Language:English
Subjects:
Alcohol
Animals
Arthropods
Biology
Blotting, Western
Bombyx - genetics
Bombyx - metabolism
Bombyx mori
Chromatography
Chromatography, Liquid
Computational Biology
Dehydrogenases
Detoxification
DNA Primers - genetics
Drosophila
Drosophila melanogaster
Electrophoresis
Electrophoresis, Gel, Two-Dimensional
Excretion
Gel electrophoresis
Genes
Genomes
Genomics
Immunity
Innate immunity
Insects
Kidneys
Laboratories
Liquid chromatography
Malpighian tubules
Malpighian Tubules - metabolism
Mass spectrometry
Mass spectroscopy
Metabolism
Organs
Osmoregulation
Proteins
Proteome - genetics
Proteome - metabolism
Proteomics
Scientific imaging
Solute transport
Tandem Mass Spectrometry
Tubules
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Summary:Malpighian tubules (MTs) are highly specific organs of arthropods (Insecta, Myriapoda and Arachnida) for excretion and osmoregulation. In order to highlight the important genes and pathways involved in multi-functions of MTs, we performed a systematic proteomic analysis of silkworm MTs in the present work. Totally, 1,367 proteins were identified by one-dimensional gel electrophoresis coupled with liquid chromatography-tandem mass spectrometry, and as well as by Trans Proteomic Pipeline (TPP) and Absolute protein expression (APEX) analyses. Forty-one proteins were further identified by two-dimensional gel electrophoresis. Some proteins were revealed to be significantly associated with various metabolic processes, organic solute transport, detoxification and innate immunity. Our results might lay a good foundation for future functional studies of MTs in silkworm and other lepidoptera.
ISSN:1932-6203
1932-6203
DOI:10.1371/journal.pone.0075731