The Sinorhizobium fredii HH103 lipopolysaccharide is not only relevant at early soybean nodulation stages but also for symbiosome stability in mature nodules

• Published in: PloS one 2013-10, Vol.8 (10), p.e74717-e74717
• Main Authors: Margaret, Isabel, Lucas, M Mercedes, Acosta-Jurado, Sebastián, Buendía-Clavería, Ana M, Fedorova, Elena, Hidalgo, Ángeles, Rodríguez-Carvajal, Miguel A, Rodriguez-Navarro, Dulce N, Ruiz-Sainz, José E, Vinardell, José M
• Format: Article
• Language: English
• Subjects: Acids; Agglutination; Bacteria; Bacteroids; Biofilms; Bradyrhizobium japonicum; Cloning; E coli; Escherichia coli; Gene mutation; Genes; Genes, Bacterial - genetics; Genetic analysis; Glycine max; Glycine max - microbiology; Glycine max - physiology; Laboratories; Legumes; Lipids; Lipopolysaccharides; Lipopolysaccharides - metabolism; Mitogens; Mutagenesis; Mutants; Mutation; Nodulation; Nodules; Phenolic compounds; Phenols; Plant Root Nodulation; Polymerase chain reaction; Sinorhizobium fredii - genetics; Sinorhizobium fredii - metabolism; Sinorhizobium fredii - physiology; Sinorhizobium meliloti; Soybeans; Symbiosis; Thick walls; Time Factors; Transcription; Transcription (Genetics); Transcription, Genetic
• ISSN: 1932-6203; 1932-6203
• DOI: 10.1371/journal.pone.0074717

In this work we have characterised the Sinorhizobium fredii HH103 greA lpsB lpsCDE genetic region and analysed for the first time the symbiotic performance of Sinorhizobium fredii lps mutants on soybean. The organization of the S. fredii HH103 greA, lpsB, and lpsCDE genes was equal to that of Sinorhizobium meliloti 1021. S. fredii HH103 greA, lpsB, and lpsE mutant derivatives produced altered LPS profiles that were characteristic of the gene mutated. In addition, S. fredii HH103 greA mutants showed a reduction in bacterial mobility and an increase of auto-agglutination in liquid cultures. RT-PCR and qPCR experiments demonstrated that the HH103 greA gene has a positive effect on the transcription of lpsB. Soybean plants inoculated with HH103 greA, lpsB or lpsE mutants formed numerous ineffective pseudonodules and showed severe symptoms of nitrogen starvation. However, HH103 greA and lps mutants were also able to induce the formation of a reduced number of soybean nodules of normal external morphology, allowing the possibility of studying the importance of bacterial LPS in later stages of the S. fredii HH103-soybean symbiosis. The infected cells of these nodules showed signs of early termination of symbiosis and lytical clearance of bacteroids. These cells also had very thick walls and accumulation of phenolic-like compounds, pointing to induced defense reactions. Our results show the importance of bacterial LPS in later stages of the S. fredii HH103-soybean symbiosis and their role in preventing host cell defense reactions. S. fredii HH103 lpsB mutants also showed reduced nodulation with Vigna unguiculata, although the symbiotic impairment was less pronounced than in soybean.