Identifying three ecological chemotypes of Xanthium strumarium glandular trichomes using a combined NMR and LC-MS method

• Published in: PloS one 2013-10, Vol.8 (10), p.e76621-e76621
• Main Authors: Chen, Fangfang, Hao, Fuhua, Li, Changfu, Gou, Junbo, Lu, Dayan, Gong, Fujun, Tang, Huiru, Zhang, Yansheng
• Format: Article
• Language: English
• Subjects: Animals; Antineoplastic Agents, Phytogenic; Artemisia annua; Asteraceae; Biosynthesis; Botanical gardens; Chromatography, High Pressure Liquid; Ecological effects; Furans - isolation & purification; Germplasm; Herbivores; Herbivory - physiology; High-performance liquid chromatography; Laboratories; Lactones; Lactones - isolation & purification; Leaves; Liquid chromatography; Localization; Magnetic Resonance Spectroscopy; Mass spectrometry; Metabolism; Metabolites; Molecular chains; Molecular physics; Natural enemies; NMR; Nuclear magnetic resonance; Pharmacology; Physics; Physiological aspects; Plant tissues; Ruminants - physiology; Secretions; Seeds; Sesquiterpene lactones; Sesquiterpenes - isolation & purification; Spectrometry, Mass, Electrospray Ionization; Staphylococcus infections; Trichomes; Trichomes - chemistry; Trichomes - classification; Trichomes - cytology; Trichomes - immunology; Xanthium; Xanthium - chemistry; Xanthium - immunology; Xanthium spinosum; Xanthium strumarium
• ISSN: 1932-6203; 1932-6203
• DOI: 10.1371/journal.pone.0076621

Xanthanolides, as the sesquiterpene lactones, are reportedly the major components for the pharmacological properties of X. strumarium L. species. Phytochemical studies indicated that the glandular structures on the surface of plant tissues would form the primary sites for the accumulation of this class of the compounds. As the interface between plants and their natural enemies, glandular trichomes may vary with respect to which of their chemicals are sequestered against different herbivores in different ecologies. However, to date, no data are available on the chemical characterisation of X. strumarium glandular cells. In this study, the trichome secretions of the X. strumarium species originating from nineteen unique areas across eleven provinces in China, were analysed by HPLC, LC-ESI-MS and NMR. For the first time three distinct chemotypes of X. strumarium glandular trichomes were discovered along with the qualitative and quantitative evaluations of their presence of xanthanolides; these were designated glandular cell Types I, II, and III, respectively. The main xanthanolides in Type I cells were 8-epi-xanthatin and xanthumin while no xanthatin was detected. Xanthatin, 8-epi-xanthatin, and xanthumin dominated in Type II cells with comparable levels of each being present. For Type III cells, significantly higher concentrations of 8-epi-xanthatin or xanthinosin (relative to xanthatin) were detected with xanthinosin only being observed in this type. Further research will focus on understanding the ecological and molecular mechanism causing these chemotype differences in X. strumarium glandular structures.