Lactobacillus acidophilus alleviates platelet-activating factor-induced inflammatory responses in human intestinal epithelial cells

Probiotics have been used as alternative prevention and therapy modalities in intestinal inflammatory disorders including inflammatory bowel diseases (IBD) and necrotizing enterocolitis (NEC). Pathophysiology of IBD and NEC includes the production of diverse lipid mediators, including platelet-activ...

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Bibliographic Details
Published in:PloS one 2013-10, Vol.8 (10), p.e75664
Main Authors: Borthakur, Alip, Bhattacharyya, Sumit, Kumar, Anoop, Anbazhagan, Arivarasu Natarajan, Tobacman, Joanne K, Dudeja, Pradeep K
Format: Article
Language:English
Subjects:
Activation
Apoptosis
Bacteria
Bcl-10 protein
Caco-2 Cells
Cell culture
Culture Media, Conditioned - pharmacology
Cytokines
Enterocolitis
Epithelial cells
Epithelial Cells - cytology
Epithelial Cells - drug effects
Epithelial Cells - immunology
Epithelial Cells - metabolism
Gastroenterology
Gastrointestinal diseases
Hepatology
Human behavior
Humans
Inflammation
Inflammatory bowel diseases
Inflammatory diseases
Interleukin 8
Interleukin-8 - metabolism
Intestine
Intestines - cytology
Kinases
Lactobacillus
Lactobacillus acidophilus
Lactobacillus acidophilus - chemistry
Medicine
Microbiota
mRNA
Necrotizing enterocolitis
NF-kappa B - metabolism
NF-κB protein
Pathogenesis
Platelet Activating Factor - pharmacology
Platelet-activating factor
Probiotics
Proteins
RNA
Rodents
Signal transduction
Small intestine
Ubiquitin
Ubiquitination
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Summary:Probiotics have been used as alternative prevention and therapy modalities in intestinal inflammatory disorders including inflammatory bowel diseases (IBD) and necrotizing enterocolitis (NEC). Pathophysiology of IBD and NEC includes the production of diverse lipid mediators, including platelet-activating factor (PAF) that mediate inflammatory responses in the disease. PAF is known to activate NF-κB, however, the mechanisms of PAF-induced inflammation are not fully defined. We have recently described a novel PAF-triggered pathway of NF-κB activation and IL-8 production in intestinal epithelial cells (IECs), requiring the pivotal role of the adaptor protein Bcl10 and its interactions with CARMA3 and MALT1. The current studies examined the potential role of the probiotic Lactobacillus acidophilus in reversing the PAF-induced, Bcl10-dependent NF-κB activation and IL-8 production in IECs. PAF treatment (5 µM×24 h) of NCM460 and Caco-2 cells significantly increased nuclear p65 NF-κB levels and IL-8 secretion (2-3-fold, P
ISSN:1932-6203
1932-6203
DOI:10.1371/journal.pone.0075664