Threonine affects intestinal function, protein synthesis and gene expression of TOR in Jian carp (Cyprinus carpio var. Jian)

This study aimed to investigate the effects of threonine (Thr) on the digestive and absorptive ability, proliferation and differentiation of enterocytes, and gene expression of juvenile Jian carp (Cyprinus carpio var. Jian). First, seven isonitrogenous diets containing graded levels of Thr (7.4-25.2...

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Published in:PloS one 2013-07, Vol.8 (7), p.e69974-e69974
Main Authors: Feng, Lin, Peng, Yan, Wu, Pei, Hu, Kai, Jiang, Wei-Dan, Liu, Yang, Jiang, Jun, Li, Shu-Hong, Zhou, Xiao-Qiu
Format: Article
Language:English
Subjects:
Absorptivity
Agriculture
Alkaline phosphatase
Ammonia
Ammonia - blood
Ammonia content
Animals
Biology
Carp
Carps - blood
Carps - physiology
Cell Differentiation
Cell Proliferation
Cells, Cultured
Cyprinus carpio jian
Diet
Differentiation
Digestion
Enterocytes
Enterocytes - cytology
Enterocytes - metabolism
Feed efficiency
Fish
Fish Proteins - genetics
Fish Proteins - metabolism
g-Glutamyltransferase
Gene expression
Gene Expression Regulation
Growth rate
Hepatopancreas
Intestine
Intestines - physiology
Kinases
Lipase
Materials Science
Medicine
Muscles
Na+/K+-exchanging ATPase
Nutrition
Protein Biosynthesis
Protein synthesis
Pyruvic acid
Regression analysis
Segments
Threonine
Threonine - metabolism
TOR Serine-Threonine Kinases - genetics
TOR Serine-Threonine Kinases - metabolism
Transaminase
Trypsin
Veterinary Science
γ-Glutamyltransferase
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cited_by cdi_FETCH-LOGICAL-c526t-33e5bb26cc579bfa795d2c8aa6471112bd5da82add2e2a7f02ca20431945a02f3
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Peng, Yan
Wu, Pei
Hu, Kai
Jiang, Wei-Dan
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Jiang, Jun
Li, Shu-Hong
Zhou, Xiao-Qiu
description This study aimed to investigate the effects of threonine (Thr) on the digestive and absorptive ability, proliferation and differentiation of enterocytes, and gene expression of juvenile Jian carp (Cyprinus carpio var. Jian). First, seven isonitrogenous diets containing graded levels of Thr (7.4-25.2 g/kg diet) were fed to the fishes for 60 days. Second, enterocyte proliferation and differentiation were assayed by culturing enterocytes with graded levels of Thr (0-275 mg/l) in vitro. Finally, enterocytes were cultured with 0 and 205 mg/l Thr to determine protein synthesis. The percent weight gain (PWG), specific growth rate, feed intake, feed efficiency, protein retention value, activities of trypsin, lipase and amylase, weights and protein contents of hepatopancreas and intestine, folds heights, activities of alkaline phosphatase (AKP), γ- glutamyl transpeptidase and Na(+)/K(+)-ATPase in all intestinal segments, glutamate-oxaloacetate transaminase (GOT) and glutamate-pyruvate transaminase (GPT) activities in hepatopancreas, and 4E-BP2 gene expression in muscle, hepatopancreas and intestinal segments were significantly enhanced by Thr (p
doi_str_mv 10.1371/journal.pone.0069974
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Jian)</title><source>PubMed Central (Open Access)</source><source>Publicly Available Content Database</source><creator>Feng, Lin ; Peng, Yan ; Wu, Pei ; Hu, Kai ; Jiang, Wei-Dan ; Liu, Yang ; Jiang, Jun ; Li, Shu-Hong ; Zhou, Xiao-Qiu</creator><contributor>Merrifield, Daniel</contributor><creatorcontrib>Feng, Lin ; Peng, Yan ; Wu, Pei ; Hu, Kai ; Jiang, Wei-Dan ; Liu, Yang ; Jiang, Jun ; Li, Shu-Hong ; Zhou, Xiao-Qiu ; Merrifield, Daniel</creatorcontrib><description>This study aimed to investigate the effects of threonine (Thr) on the digestive and absorptive ability, proliferation and differentiation of enterocytes, and gene expression of juvenile Jian carp (Cyprinus carpio var. Jian). First, seven isonitrogenous diets containing graded levels of Thr (7.4-25.2 g/kg diet) were fed to the fishes for 60 days. Second, enterocyte proliferation and differentiation were assayed by culturing enterocytes with graded levels of Thr (0-275 mg/l) in vitro. Finally, enterocytes were cultured with 0 and 205 mg/l Thr to determine protein synthesis. The percent weight gain (PWG), specific growth rate, feed intake, feed efficiency, protein retention value, activities of trypsin, lipase and amylase, weights and protein contents of hepatopancreas and intestine, folds heights, activities of alkaline phosphatase (AKP), γ- glutamyl transpeptidase and Na(+)/K(+)-ATPase in all intestinal segments, glutamate-oxaloacetate transaminase (GOT) and glutamate-pyruvate transaminase (GPT) activities in hepatopancreas, and 4E-BP2 gene expression in muscle, hepatopancreas and intestinal segments were significantly enhanced by Thr (p&lt;0.05). However, the plasma ammonia concentration and TOR gene expression decreased (p&lt;0.05). In vitro, Thr supplement significantly increased cell numbers, protein content, the activities of GOT, GPT, AKP and Na(+)/K(+)-ATPase, and protein synthesis rate of enterocytes, and decreased LDH activity and ammonia content in cell medium (p&lt;0.05). In conclusion, Thr improved growth, digestive and absorptive capacity, enterocyte proliferation and differentiation, and protein synthesis and regulated TOR and 4E-BP2 gene expression in juvenile Jian carp. 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Jian)</atitle><jtitle>PloS one</jtitle><addtitle>PLoS One</addtitle><date>2013-07-26</date><risdate>2013</risdate><volume>8</volume><issue>7</issue><spage>e69974</spage><epage>e69974</epage><pages>e69974-e69974</pages><issn>1932-6203</issn><eissn>1932-6203</eissn><abstract>This study aimed to investigate the effects of threonine (Thr) on the digestive and absorptive ability, proliferation and differentiation of enterocytes, and gene expression of juvenile Jian carp (Cyprinus carpio var. Jian). First, seven isonitrogenous diets containing graded levels of Thr (7.4-25.2 g/kg diet) were fed to the fishes for 60 days. Second, enterocyte proliferation and differentiation were assayed by culturing enterocytes with graded levels of Thr (0-275 mg/l) in vitro. Finally, enterocytes were cultured with 0 and 205 mg/l Thr to determine protein synthesis. The percent weight gain (PWG), specific growth rate, feed intake, feed efficiency, protein retention value, activities of trypsin, lipase and amylase, weights and protein contents of hepatopancreas and intestine, folds heights, activities of alkaline phosphatase (AKP), γ- glutamyl transpeptidase and Na(+)/K(+)-ATPase in all intestinal segments, glutamate-oxaloacetate transaminase (GOT) and glutamate-pyruvate transaminase (GPT) activities in hepatopancreas, and 4E-BP2 gene expression in muscle, hepatopancreas and intestinal segments were significantly enhanced by Thr (p&lt;0.05). However, the plasma ammonia concentration and TOR gene expression decreased (p&lt;0.05). In vitro, Thr supplement significantly increased cell numbers, protein content, the activities of GOT, GPT, AKP and Na(+)/K(+)-ATPase, and protein synthesis rate of enterocytes, and decreased LDH activity and ammonia content in cell medium (p&lt;0.05). In conclusion, Thr improved growth, digestive and absorptive capacity, enterocyte proliferation and differentiation, and protein synthesis and regulated TOR and 4E-BP2 gene expression in juvenile Jian carp. The dietary Thr requirement of juvenile Jian carp was 16.25 g/kg diet (51.3 g/kg protein) based on quadratic regression analysis of PWG.</abstract><cop>United States</cop><pub>Public Library of Science</pub><pmid>23922879</pmid><doi>10.1371/journal.pone.0069974</doi><oa>free_for_read</oa></addata></record>
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subjects Absorptivity
Agriculture
Alkaline phosphatase
Ammonia
Ammonia - blood
Ammonia content
Animals
Biology
Carp
Carps - blood
Carps - physiology
Cell Differentiation
Cell Proliferation
Cells, Cultured
Cyprinus carpio jian
Diet
Differentiation
Digestion
Enterocytes
Enterocytes - cytology
Enterocytes - metabolism
Feed efficiency
Fish
Fish Proteins - genetics
Fish Proteins - metabolism
g-Glutamyltransferase
Gene expression
Gene Expression Regulation
Growth rate
Hepatopancreas
Intestine
Intestines - physiology
Kinases
Lipase
Materials Science
Medicine
Muscles
Na+/K+-exchanging ATPase
Nutrition
Protein Biosynthesis
Protein synthesis
Pyruvic acid
Regression analysis
Segments
Threonine
Threonine - metabolism
TOR Serine-Threonine Kinases - genetics
TOR Serine-Threonine Kinases - metabolism
Transaminase
Trypsin
Veterinary Science
γ-Glutamyltransferase
title Threonine affects intestinal function, protein synthesis and gene expression of TOR in Jian carp (Cyprinus carpio var. Jian)
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