Characterization of lung cancer by amide proton transfer (APT) imaging: an in-vivo study in an orthotopic mouse model

Amide proton transfer (APT) imaging is one of the chemical exchange saturation transfer (CEST) imaging methods which images the exchange between protons of free tissue water and the amide groups (-NH) of endogenous mobile proteins and peptides. Previous work suggested the ability of APT imaging for...

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Bibliographic Details
Published in:PloS one 2013-10, Vol.8 (10), p.e77019
Main Authors: Togao, Osamu, Kessinger, Chase W, Huang, Gang, Soesbe, Todd C, Sagiyama, Koji, Dimitrov, Ivan, Sherry, A Dean, Gao, Jinming, Takahashi, Masaya
Format: Article
Language:English
Subjects:
Amides - chemistry
Animals
Biopsy
Brain
Brain cancer
Brain research
Brain tumors
Cancer
Cancer research
Cell Line, Tumor
Cell Transformation, Neoplastic
Disease Models, Animal
Feasibility studies
Female
Humans
Invasiveness
Lung cancer
Lung diseases
Lung Neoplasms - diagnosis
Lung Neoplasms - pathology
Medical imaging
Medical prognosis
Medical research
Mice
Molecular Imaging - methods
Neuroimaging
NMR
Nuclear magnetic resonance
Peptides
Pharmacology
Proteins
Protons
Respiratory-Gated Imaging Techniques
Tumor cell lines
Tumors
Water - chemistry
Xenografts
Xenotransplantation
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Summary:Amide proton transfer (APT) imaging is one of the chemical exchange saturation transfer (CEST) imaging methods which images the exchange between protons of free tissue water and the amide groups (-NH) of endogenous mobile proteins and peptides. Previous work suggested the ability of APT imaging for characterization of the tumoral grade in the brain tumor. In this study, we tested the feasibility of in-vivo APT imaging of lung tumor and investigated whether the method could differentiate the tumoral types on orthotopic tumor xenografts from two malignant lung cancer cell lines. The results revealed that APT imaging is feasible to quantify lung tumors in the moving lung. The measured APT effect was higher in the tumor which exhibited more active proliferation than the other. The present study demonstrates that APT imaging has the potential to provide a characterization test to differentiate types or grade of lung cancer noninvasively, which may eventually reduce the need invasive needle biopsy or resection for lung cancer.
ISSN:1932-6203
1932-6203
DOI:10.1371/journal.pone.0077019