Microarray analysis of microbiota of gingival lesions in noma patients

Noma (cancrum oris) is a gangrenous disease of unknown etiology affecting the maxillo-facial region of young children in extremely limited resource countries. In an attempt to better understand the microbiological events occurring during this disease, we used phylogenetic and low-density microarrays...

Full description

Saved in:
Bibliographic Details
Published in:PLoS neglected tropical diseases 2013-09, Vol.7 (9), p.e2453-e2453
Main Authors: Huyghe, Antoine, François, Patrice, Mombelli, Andrea, Tangomo, Manuela, Girard, Myriam, Baratti-Mayer, Denise, Bolivar, Ignacio, Pittet, Didier, Schrenzel, Jacques
Format: Article
Language:English
Subjects:
Bacteria - classification
Bacteria - genetics
Bacterial infections
Capnocytophaga
Child
Child, Preschool
Disease
Female
Fusobacterium necrophorum
Gangrene
Genetic aspects
Host-bacteria relationships
Humans
Hygiene
Identification and classification
Infant
Infections
Male
Malnutrition
Microarray Analysis
Microbiota
Mortality
Noma - microbiology
Peptostreptococcus
Phylogeny
Porphyromonas
Prevotella intermedia
RNA
RNA, Ribosomal, 16S - genetics
Streptococcus anginosus
Streptococcus pyogenes
Taxonomy
Testing
Viral infections
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Noma (cancrum oris) is a gangrenous disease of unknown etiology affecting the maxillo-facial region of young children in extremely limited resource countries. In an attempt to better understand the microbiological events occurring during this disease, we used phylogenetic and low-density microarrays targeting the 16S rRNA gene to characterize the gingival flora of acute noma and acute necrotizing gingivitis (ANG) lesions, and compared them to healthy control subjects of the same geographical and social background. Our observations raise doubts about Fusobacterium necrophorum, a previously suspected causative agent of noma, as this species was not associated with noma lesions. Various oral pathogens were more abundant in noma lesions, notably Atopobium spp., Prevotella intermedia, Peptostreptococcus spp., Streptococcus pyogenes and Streptococcus anginosus. On the other hand, pathogens associated with periodontal diseases such as Aggregatibacter actinomycetemcomitans, Capnocytophaga spp., Porphyromonas spp. and Fusobacteriales were more abundant in healthy controls. Importantly, the overall loss of bacterial diversity observed in noma samples as well as its homology to that of ANG microbiota supports the hypothesis that ANG might be the immediate step preceding noma.
ISSN:1935-2735
1935-2727
1935-2735
DOI:10.1371/journal.pntd.0002453