Transcriptional response to deletion of the phosphatidylserine decarboxylase Psd1p in the yeast Saccharomyces cerevisiae

• Published in: PloS one 2013-10, Vol.8 (10), p.e77380
• Main Authors: Gsell, Martina, Mascher, Gerald, Schuiki, Irmgard, Ploier, Birgit, Hrastnik, Claudia, Daum, Günther
• Format: Article
• Language: English
• Subjects: Baking yeast; Biochemistry; Biosynthesis; Carboxy-Lyases - genetics; Carboxy-Lyases - metabolism; Cell Membrane - metabolism; Clonal deletion; Cloning; Comparative analysis; Deletion mutant; Deoxyribonucleic acid; DNA; DNA microarrays; Enzymes; Gene Deletion; Gene expression; Gene Expression Regulation, Fungal; Gene regulation; Genes; Genetic aspects; Genomes; Genomics; Intracellular Space; Lipids; Membranes; Metabolism; Mitochondria; Mitochondria - metabolism; Mitochondrial Proteins - genetics; Mitochondrial Proteins - metabolism; Mutants; Mutation; Open Reading Frames; Phenotype; Phenotypes; Phosphatidylethanolamine; Phosphatidylserine; Phosphatidylserine decarboxylase; Phosphatidylserine decarboxylase 1; Phospholipids; Phospholipids - metabolism; Protein Transport; Proteins; Saccharomyces cerevisiae; Saccharomyces cerevisiae - genetics; Saccharomyces cerevisiae - growth & development; Saccharomyces cerevisiae - metabolism; Saccharomyces cerevisiae Proteins - genetics; Saccharomyces cerevisiae Proteins - metabolism; Transcription; Transcription (Genetics); Transcription, Genetic; Yeast
• ISSN: 1932-6203; 1932-6203
• DOI: 10.1371/journal.pone.0077380

In the yeast, Saccharomyces cerevisiae, the synthesis of the essential phospholipid phosphatidylethanolamine (PE) is accomplished by a network of reactions which comprises four different pathways. The enzyme contributing most to PE formation is the mitochondrial phosphatidylserine decarboxylase 1 (Psd1p) which catalyzes conversion of phosphatidylserine (PS) to PE. To study the genome wide effect of an unbalanced cellular and mitochondrial PE level and in particular the contribution of Psd1p to this depletion we performed a DNA microarray analysis with a ∆psd1 deletion mutant. This approach revealed that 54 yeast genes were significantly up-regulated in the absence of PSD1 compared to wild type. Surprisingly, marked down-regulation of genes was not observed. A number of different cellular processes in different subcellular compartments were affected in a ∆psd1 mutant. Deletion mutants bearing defects in all 54 candidate genes, respectively, were analyzed for their growth phenotype and their phospholipid profile. Only three mutants, namely ∆gpm2, ∆gph1 and ∆rsb1, were affected in one of these parameters. The possible link of these mutations to PE deficiency and PSD1 deletion is discussed.