Sialoglycoproteins and N-glycans from secreted exosomes of ovarian carcinoma cells

Exosomes consist of vesicles that are secreted by several human cells, including tumor cells and neurons, and they are found in several biological fluids. Exosomes have characteristic protein and lipid composition, however, the results concerning glycoprotein composition and glycosylation are scarce...

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Bibliographic Details
Published in:PloS one 2013-10, Vol.8 (10), p.e78631-e78631
Main Authors: Escrevente, Cristina, Grammel, Nicolas, Kandzia, Sebastian, Zeiser, Johannes, Tranfield, Erin M, Conradt, Harald S, Costa, Júlia
Format: Article
Language:English
Subjects:
Antigens, Neoplasm - metabolism
Biomarkers, Tumor - metabolism
Biomarkers, Tumor - secretion
Biosynthesis
Carbohydrate Conformation
Carbohydrate Sequence
Carrier Proteins - metabolism
Carrier Proteins - secretion
Cell Line, Tumor
Chromatography
Dendritic cells
Exosomes
Exosomes - metabolism
Exosomes - secretion
Female
Fingerprinting
Fucose
Galectin-3
Glycan
Glycoprotein composition
Glycoproteins
Glycoproteins - metabolism
Glycoproteins - secretion
Glycosylation
High-performance liquid chromatography
Humans
Laboratories
Lectins
Lipid composition
Lipids
Liquid chromatography
Mannans - metabolism
Mannans - secretion
Mannose
Mass spectrometry
Mass spectroscopy
Microscopy
Molecular Sequence Data
N-Acetylglucosamine
N-glycans
Ovarian cancer
Ovarian carcinoma
Ovarian Neoplasms
Peptide mapping
Plasma
Polysaccharides
Polysaccharides - metabolism
Polysaccharides - secretion
Protein composition
Proteins
Proteomics
Sialoglycoproteins
Sialoglycoproteins - metabolism
Sialoglycoproteins - secretion
Streptococcus infections
Tumor cells
Urine
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Summary:Exosomes consist of vesicles that are secreted by several human cells, including tumor cells and neurons, and they are found in several biological fluids. Exosomes have characteristic protein and lipid composition, however, the results concerning glycoprotein composition and glycosylation are scarce. Here, protein glycosylation of exosomes from ovarian carcinoma SKOV3 cells has been studied by lectin blotting, NP-HPLC analysis of 2-aminobenzamide labeled glycans and mass spectrometry. An abundant sialoglycoprotein was found enriched in exosomes and it was identified by peptide mass fingerprinting and immunoblot as the galectin-3-binding protein (LGALS3BP). Exosomes were found to contain predominantly complex glycans of the di-, tri-, and tetraantennary type with or without proximal fucose and also high mannose glycans. Diantennary glycans containing bisecting N-acetylglucosamine were also detected. This work provides detailed information about glycoprotein and N-glycan composition of exosomes from ovarian cancer cells, furthermore it opens novel perspectives to further explore the functional role of glycans in the biology of exosomes.
ISSN:1932-6203
1932-6203
DOI:10.1371/journal.pone.0078631