Develop to term rat oocytes injected with heat-dried sperm heads

• Published in: PloS one 2013-11, Vol.8 (11), p.e78260-e78260
• Main Authors: Lee, Kyung-Bon, Park, Ki-Eun, Kwon, In-Kiu, Tripurani, Swamy K, Kim, Keun Jung, Lee, Ji Hye, Niwa, Koji, Kim, Min Kyu
• Format: Article
• Language: English
• Subjects: Air; Animal sciences; Animals; Biotechnology; Blastocyst - cytology; Blastocyst - physiology; Cattle; Cell cycle; Cleavage; Desiccation; Drying; Embryo Implantation; Embryonic development; Embryos; Female; Freeze Drying; Heat; Hot Temperature; In vivo methods and tests; Laboratories; Life sciences; Male; Morula - cytology; Morula - physiology; Motility; Nitrogen; Nitrogen (Chemical element); Oocytes; Oocytes - cytology; Oocytes - physiology; Rats; Rats, Wistar; Semen Preservation; Sperm; Sperm Head - physiology; Sperm Injections, Intracytoplasmic; Spermatozoa; Vapor phases; Zoology
• ISSN: 1932-6203; 1932-6203
• DOI: 10.1371/journal.pone.0078260

This study investigated the development of rat oocytes in vitro and in vivo following intracytoplasmic injection of heads from spermatozoa heat-dried at 50°C for 8 h and stored at 4°C in different gas phases. Sperm membrane and chromosome are damaged by the process of heat-drying. Oocyte activation and cleavage of oocytes were worse in oocytes injected with spermatozoa heat-dried and stored for 1 week than unheated, fresh spermatozoa, but in heat-dried spermatozoa, there were no differences in these abilities of oocytes between the samples stored in nitrogen gas and in air. The oocytes injected with heat-dried spermatozoa stored for 1 week could develop to the morula and blastocyst stages without difference between the samples stored in nitrogen gas and in air after artificial stimulation. Cleavage of oocytes and development of cleaved embryos were higher when heat-dried spermatozoa were stored for 3 and 6 months in nitrogen gas than in air. However, the ability of injected oocytes to develop to the morula and blastocyst stages was not inhibited even when heat-dried spermatozoa stored in both atmosphere conditions for as long as 6 months were used. When 2-cell embryos derived from oocytes injected with heads from spermatozoa heat-dried and stored for 1 week and 1 month were transferred, each 1 of 4 recipients was conceived, and the conceived recipients delivered 1 live young each. These results demonstrate that rat oocytes can be fertilized with heat-dried spermatozoa and that the fertilized oocytes can develop to term.