Develop to term rat oocytes injected with heat-dried sperm heads

This study investigated the development of rat oocytes in vitro and in vivo following intracytoplasmic injection of heads from spermatozoa heat-dried at 50°C for 8 h and stored at 4°C in different gas phases. Sperm membrane and chromosome are damaged by the process of heat-drying. Oocyte activation...

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Published in:PloS one 2013-11, Vol.8 (11), p.e78260-e78260
Main Authors: Lee, Kyung-Bon, Park, Ki-Eun, Kwon, In-Kiu, Tripurani, Swamy K, Kim, Keun Jung, Lee, Ji Hye, Niwa, Koji, Kim, Min Kyu
Format: Article
Language:English
Subjects:
Air
Animal sciences
Animals
Biotechnology
Blastocyst - cytology
Blastocyst - physiology
Cattle
Cell cycle
Cleavage
Desiccation
Drying
Embryo Implantation
Embryonic development
Embryos
Female
Freeze Drying
Heat
Hot Temperature
In vivo methods and tests
Laboratories
Life sciences
Male
Morula - cytology
Morula - physiology
Motility
Nitrogen
Nitrogen (Chemical element)
Oocytes
Oocytes - cytology
Oocytes - physiology
Rats
Rats, Wistar
Semen Preservation
Sperm
Sperm Head - physiology
Sperm Injections, Intracytoplasmic
Spermatozoa
Vapor phases
Zoology
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cited_by cdi_FETCH-LOGICAL-c692t-297d8904dacb96d917e458b1e2a1b495aebe50e0ff380bf4f2fa0633026725173
cites cdi_FETCH-LOGICAL-c692t-297d8904dacb96d917e458b1e2a1b495aebe50e0ff380bf4f2fa0633026725173
container_end_page e78260
container_issue 11
container_start_page e78260
container_title PloS one
container_volume 8
creator Lee, Kyung-Bon
Park, Ki-Eun
Kwon, In-Kiu
Tripurani, Swamy K
Kim, Keun Jung
Lee, Ji Hye
Niwa, Koji
Kim, Min Kyu
description This study investigated the development of rat oocytes in vitro and in vivo following intracytoplasmic injection of heads from spermatozoa heat-dried at 50°C for 8 h and stored at 4°C in different gas phases. Sperm membrane and chromosome are damaged by the process of heat-drying. Oocyte activation and cleavage of oocytes were worse in oocytes injected with spermatozoa heat-dried and stored for 1 week than unheated, fresh spermatozoa, but in heat-dried spermatozoa, there were no differences in these abilities of oocytes between the samples stored in nitrogen gas and in air. The oocytes injected with heat-dried spermatozoa stored for 1 week could develop to the morula and blastocyst stages without difference between the samples stored in nitrogen gas and in air after artificial stimulation. Cleavage of oocytes and development of cleaved embryos were higher when heat-dried spermatozoa were stored for 3 and 6 months in nitrogen gas than in air. However, the ability of injected oocytes to develop to the morula and blastocyst stages was not inhibited even when heat-dried spermatozoa stored in both atmosphere conditions for as long as 6 months were used. When 2-cell embryos derived from oocytes injected with heads from spermatozoa heat-dried and stored for 1 week and 1 month were transferred, each 1 of 4 recipients was conceived, and the conceived recipients delivered 1 live young each. These results demonstrate that rat oocytes can be fertilized with heat-dried spermatozoa and that the fertilized oocytes can develop to term.
doi_str_mv 10.1371/journal.pone.0078260
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The oocytes injected with heat-dried spermatozoa stored for 1 week could develop to the morula and blastocyst stages without difference between the samples stored in nitrogen gas and in air after artificial stimulation. Cleavage of oocytes and development of cleaved embryos were higher when heat-dried spermatozoa were stored for 3 and 6 months in nitrogen gas than in air. However, the ability of injected oocytes to develop to the morula and blastocyst stages was not inhibited even when heat-dried spermatozoa stored in both atmosphere conditions for as long as 6 months were used. When 2-cell embryos derived from oocytes injected with heads from spermatozoa heat-dried and stored for 1 week and 1 month were transferred, each 1 of 4 recipients was conceived, and the conceived recipients delivered 1 live young each. 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Oocyte activation and cleavage of oocytes were worse in oocytes injected with spermatozoa heat-dried and stored for 1 week than unheated, fresh spermatozoa, but in heat-dried spermatozoa, there were no differences in these abilities of oocytes between the samples stored in nitrogen gas and in air. The oocytes injected with heat-dried spermatozoa stored for 1 week could develop to the morula and blastocyst stages without difference between the samples stored in nitrogen gas and in air after artificial stimulation. Cleavage of oocytes and development of cleaved embryos were higher when heat-dried spermatozoa were stored for 3 and 6 months in nitrogen gas than in air. However, the ability of injected oocytes to develop to the morula and blastocyst stages was not inhibited even when heat-dried spermatozoa stored in both atmosphere conditions for as long as 6 months were used. 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Steven</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Develop to term rat oocytes injected with heat-dried sperm heads</atitle><jtitle>PloS one</jtitle><addtitle>PLoS One</addtitle><date>2013-11-04</date><risdate>2013</risdate><volume>8</volume><issue>11</issue><spage>e78260</spage><epage>e78260</epage><pages>e78260-e78260</pages><issn>1932-6203</issn><eissn>1932-6203</eissn><abstract>This study investigated the development of rat oocytes in vitro and in vivo following intracytoplasmic injection of heads from spermatozoa heat-dried at 50°C for 8 h and stored at 4°C in different gas phases. Sperm membrane and chromosome are damaged by the process of heat-drying. Oocyte activation and cleavage of oocytes were worse in oocytes injected with spermatozoa heat-dried and stored for 1 week than unheated, fresh spermatozoa, but in heat-dried spermatozoa, there were no differences in these abilities of oocytes between the samples stored in nitrogen gas and in air. The oocytes injected with heat-dried spermatozoa stored for 1 week could develop to the morula and blastocyst stages without difference between the samples stored in nitrogen gas and in air after artificial stimulation. Cleavage of oocytes and development of cleaved embryos were higher when heat-dried spermatozoa were stored for 3 and 6 months in nitrogen gas than in air. However, the ability of injected oocytes to develop to the morula and blastocyst stages was not inhibited even when heat-dried spermatozoa stored in both atmosphere conditions for as long as 6 months were used. When 2-cell embryos derived from oocytes injected with heads from spermatozoa heat-dried and stored for 1 week and 1 month were transferred, each 1 of 4 recipients was conceived, and the conceived recipients delivered 1 live young each. These results demonstrate that rat oocytes can be fertilized with heat-dried spermatozoa and that the fertilized oocytes can develop to term.</abstract><cop>United States</cop><pub>Public Library of Science</pub><pmid>24223784</pmid><doi>10.1371/journal.pone.0078260</doi><tpages>e78260</tpages><oa>free_for_read</oa></addata></record>
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source Publicly Available Content Database (Proquest) (PQ_SDU_P3); PubMed Central
subjects Air
Animal sciences
Animals
Biotechnology
Blastocyst - cytology
Blastocyst - physiology
Cattle
Cell cycle
Cleavage
Desiccation
Drying
Embryo Implantation
Embryonic development
Embryos
Female
Freeze Drying
Heat
Hot Temperature
In vivo methods and tests
Laboratories
Life sciences
Male
Morula - cytology
Morula - physiology
Motility
Nitrogen
Nitrogen (Chemical element)
Oocytes
Oocytes - cytology
Oocytes - physiology
Rats
Rats, Wistar
Semen Preservation
Sperm
Sperm Head - physiology
Sperm Injections, Intracytoplasmic
Spermatozoa
Vapor phases
Zoology
title Develop to term rat oocytes injected with heat-dried sperm heads
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