Biological, functional and genetic characterization of bone marrow-derived mesenchymal stromal cells from pediatric patients affected by acute lymphoblastic leukemia

Alterations in hematopoietic microenvironment of acute lymphoblastic leukemia patients have been claimed to occur, but little is known about the components of marrow stroma in these patients. In this study, we characterized mesenchymal stromal cells (MSCs) isolated from bone marrow (BM) of 45 pediat...

Full description

Saved in:
Bibliographic Details
Published in:PloS one 2013-11, Vol.8 (11), p.e76989
Main Authors: Conforti, Antonella, Biagini, Simone, Del Bufalo, Francesca, Sirleto, Pietro, Angioni, Adriano, Starc, Nadia, Li Pira, Giuseppina, Moretta, Francesca, Proia, Alessandra, Contoli, Benedetta, Genovese, Silvia, Ciardi, Claudia, Avanzini, Maria Antonietta, Rosti, Vittorio, Lo-Coco, Francesco, Locatelli, Franco, Bernardo, Maria Ester
Format: Article
Language:English
Subjects:
Abnormalities
Acute lymphoblastic leukemia
Acute lymphocytic leukemia
Adolescent
Analysis
Anemia
Antineoplastic Combined Chemotherapy Protocols - administration & dosage
Blood diseases
Bone marrow
Bone Marrow Cells - metabolism
Bone Marrow Cells - pathology
Cancer
Cell culture
Cell Differentiation - drug effects
Cell proliferation
Cells, Cultured
Chemotherapy
Child
Child health
Child, Preschool
Children & youth
Chromosomes
Cytogenetics
Diagnosis
Differentiation
Disease
Genetic abnormalities
Genetic aspects
Genetics
Hematology
Hematopoiesis
Hematopoiesis - drug effects
Hospitals
Humans
Immunomodulation
Infant
Laboratories
Leukemia
Lymphatic leukemia
Lymphocytes T
Male
Medical research
Mesenchymal stem cells
Mesenchymal Stromal Cells - metabolism
Mesenchymal Stromal Cells - pathology
Mesenchyme
Morphology
Oncology
Patients
Pediatrics
Precursor Cell Lymphoblastic Leukemia-Lymphoma - drug therapy
Precursor Cell Lymphoblastic Leukemia-Lymphoma - metabolism
Precursor Cell Lymphoblastic Leukemia-Lymphoma - pathology
Stem cells
Stromal cells
T cells
Time Factors
Translocation
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
cited_by cdi_FETCH-LOGICAL-c758t-89ef69d2a98acfecc8d5976b39e99c880a660a0cb9910fc3e866021a66475fbc3
cites cdi_FETCH-LOGICAL-c758t-89ef69d2a98acfecc8d5976b39e99c880a660a0cb9910fc3e866021a66475fbc3
container_end_page
container_issue 11
container_start_page e76989
container_title PloS one
container_volume 8
creator Conforti, Antonella
Biagini, Simone
Del Bufalo, Francesca
Sirleto, Pietro
Angioni, Adriano
Starc, Nadia
Li Pira, Giuseppina
Moretta, Francesca
Proia, Alessandra
Contoli, Benedetta
Genovese, Silvia
Ciardi, Claudia
Avanzini, Maria Antonietta
Rosti, Vittorio
Lo-Coco, Francesco
Locatelli, Franco
Bernardo, Maria Ester
description Alterations in hematopoietic microenvironment of acute lymphoblastic leukemia patients have been claimed to occur, but little is known about the components of marrow stroma in these patients. In this study, we characterized mesenchymal stromal cells (MSCs) isolated from bone marrow (BM) of 45 pediatric patients with acute lymphoblastic leukemia (ALL-MSCs) at diagnosis (day+0) and during chemotherapy treatment (days: +15; +33; +78), the time points being chosen according to the schedule of BM aspirates required by the AIEOP-BFM ALL 2009 treatment protocol. Morphology, proliferative capacity, immunophenotype, differentiation potential, immunomodulatory properties and ability to support long-term hematopoiesis of ALL-MSCs were analysed and compared with those from 41 healthy donors (HD-MSCs). ALL-MSCs were also genetically characterized through array-CGH, conventional karyotyping and FISH analysis. Moreover, we compared ALL-MSCs generated at day+0 with those isolated during chemotherapy. Morphology, immunophenotype, differentiation potential and in vitro life-span did not differ between ALL-MSCs and HD-MSCs. ALL-MSCs showed significantly lower proliferative capacity (p
doi_str_mv 10.1371/journal.pone.0076989
format article
fullrecord <record><control><sourceid>gale_plos_</sourceid><recordid>TN_cdi_plos_journals_1449331977</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><galeid>A478392487</galeid><doaj_id>oai_doaj_org_article_ca9448cc504347e6a3a6100ba6e66958</doaj_id><sourcerecordid>A478392487</sourcerecordid><originalsourceid>FETCH-LOGICAL-c758t-89ef69d2a98acfecc8d5976b39e99c880a660a0cb9910fc3e866021a66475fbc3</originalsourceid><addsrcrecordid>eNqNk22L1DAQx4so3nn6DUQDgiC4a9qkafJGOA8fFg4OfHobpul0N2fa7CXt6fp9_J6m7t6xCwrSF2lnfvOf4Z9Olj3O6TxnVf7q0o-hBzdf-x7nlFZCSXUnO84VK2aioOzu3vtR9iDGS0pLJoW4nx0VvOC8qPLj7Ncb651fWgPuJWnH3gzWJ1UCfUOW2ONgDTErCGAGDPYnTGniW1KnrqSDEPz3WZMy19iQDiP2ZrXpUn0cgp9Og85F0qYPssbGwhCS4DrJYD9EAm2LSbgh9YaAGQckbtOtV752EKfODsdv2Fl4mN1rwUV8tDtPsi_v3n4--zA7v3i_ODs9n5mqlMNMKmyFagpQEkxSNrIpVSVqplApIyUFIShQUyuV09YwTG7QIk9RXpVtbdhJ9nSru3Y-6p3DUeecK8ZyVVWJWGyJxsOlXgebPNhoD1b_Cfiw1BDS6A61AcW5NKaknPEKBTAQOaU1CBRClTJpvd51G-sOG5MsCeAORA8zvV3ppb_WTBZUVGUSeLYTCP5qxDj8Y-QdtYQ0le1bn8RMZ6PRp7ySTBVcTtT8L1R6mnQBJt12a1P8oODFQUFiBvwxLGGMUS8-ffx_9uLrIft8j10huGEVvRunPy8egnwLmuBjDNjeOpdTPa3IjRt6WhG9W5FU9mTf9duim51gvwHt1hDB</addsrcrecordid><sourcetype>Open Website</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>1449331977</pqid></control><display><type>article</type><title>Biological, functional and genetic characterization of bone marrow-derived mesenchymal stromal cells from pediatric patients affected by acute lymphoblastic leukemia</title><source>PubMed Central Free</source><source>Publicly Available Content Database</source><creator>Conforti, Antonella ; Biagini, Simone ; Del Bufalo, Francesca ; Sirleto, Pietro ; Angioni, Adriano ; Starc, Nadia ; Li Pira, Giuseppina ; Moretta, Francesca ; Proia, Alessandra ; Contoli, Benedetta ; Genovese, Silvia ; Ciardi, Claudia ; Avanzini, Maria Antonietta ; Rosti, Vittorio ; Lo-Coco, Francesco ; Locatelli, Franco ; Bernardo, Maria Ester</creator><contributor>Menendez, Pablo</contributor><creatorcontrib>Conforti, Antonella ; Biagini, Simone ; Del Bufalo, Francesca ; Sirleto, Pietro ; Angioni, Adriano ; Starc, Nadia ; Li Pira, Giuseppina ; Moretta, Francesca ; Proia, Alessandra ; Contoli, Benedetta ; Genovese, Silvia ; Ciardi, Claudia ; Avanzini, Maria Antonietta ; Rosti, Vittorio ; Lo-Coco, Francesco ; Locatelli, Franco ; Bernardo, Maria Ester ; Menendez, Pablo</creatorcontrib><description>Alterations in hematopoietic microenvironment of acute lymphoblastic leukemia patients have been claimed to occur, but little is known about the components of marrow stroma in these patients. In this study, we characterized mesenchymal stromal cells (MSCs) isolated from bone marrow (BM) of 45 pediatric patients with acute lymphoblastic leukemia (ALL-MSCs) at diagnosis (day+0) and during chemotherapy treatment (days: +15; +33; +78), the time points being chosen according to the schedule of BM aspirates required by the AIEOP-BFM ALL 2009 treatment protocol. Morphology, proliferative capacity, immunophenotype, differentiation potential, immunomodulatory properties and ability to support long-term hematopoiesis of ALL-MSCs were analysed and compared with those from 41 healthy donors (HD-MSCs). ALL-MSCs were also genetically characterized through array-CGH, conventional karyotyping and FISH analysis. Moreover, we compared ALL-MSCs generated at day+0 with those isolated during chemotherapy. Morphology, immunophenotype, differentiation potential and in vitro life-span did not differ between ALL-MSCs and HD-MSCs. ALL-MSCs showed significantly lower proliferative capacity (p&lt;0.001) and ability to support in vitro hematopoiesis (p = 0.04) as compared with HD-MSCs, while they had similar capacity to inhibit in vitro mitogen-induced T-cell proliferation (p = N.S.). ALL-MSCs showed neither the typical translocations carried by the leukemic clone (when present), nor other genetic abnormalities acquired during ex vivo culture. Our findings indicate that ALL-MSCs display reduced ability to proliferate and to support long-term hematopoiesis in vitro. ALL-MSCs isolated at diagnosis do not differ from those obtained during treatment.</description><identifier>ISSN: 1932-6203</identifier><identifier>EISSN: 1932-6203</identifier><identifier>DOI: 10.1371/journal.pone.0076989</identifier><identifier>PMID: 24244271</identifier><language>eng</language><publisher>United States: Public Library of Science</publisher><subject>Abnormalities ; Acute lymphoblastic leukemia ; Acute lymphocytic leukemia ; Adolescent ; Analysis ; Anemia ; Antineoplastic Combined Chemotherapy Protocols - administration &amp; dosage ; Blood diseases ; Bone marrow ; Bone Marrow Cells - metabolism ; Bone Marrow Cells - pathology ; Cancer ; Cell culture ; Cell Differentiation - drug effects ; Cell proliferation ; Cells, Cultured ; Chemotherapy ; Child ; Child health ; Child, Preschool ; Children &amp; youth ; Chromosomes ; Cytogenetics ; Diagnosis ; Differentiation ; Disease ; Genetic abnormalities ; Genetic aspects ; Genetics ; Hematology ; Hematopoiesis ; Hematopoiesis - drug effects ; Hospitals ; Humans ; Immunomodulation ; Infant ; Laboratories ; Leukemia ; Lymphatic leukemia ; Lymphocytes T ; Male ; Medical research ; Mesenchymal stem cells ; Mesenchymal Stromal Cells - metabolism ; Mesenchymal Stromal Cells - pathology ; Mesenchyme ; Morphology ; Oncology ; Patients ; Pediatrics ; Precursor Cell Lymphoblastic Leukemia-Lymphoma - drug therapy ; Precursor Cell Lymphoblastic Leukemia-Lymphoma - metabolism ; Precursor Cell Lymphoblastic Leukemia-Lymphoma - pathology ; Stem cells ; Stromal cells ; T cells ; Time Factors ; Translocation</subject><ispartof>PloS one, 2013-11, Vol.8 (11), p.e76989</ispartof><rights>COPYRIGHT 2013 Public Library of Science</rights><rights>2013 Conforti et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License: https://creativecommons.org/licenses/by/4.0/ (the “License”), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><rights>2013 Conforti et al 2013 Conforti et al</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c758t-89ef69d2a98acfecc8d5976b39e99c880a660a0cb9910fc3e866021a66475fbc3</citedby><cites>FETCH-LOGICAL-c758t-89ef69d2a98acfecc8d5976b39e99c880a660a0cb9910fc3e866021a66475fbc3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.proquest.com/docview/1449331977/fulltextPDF?pq-origsite=primo$$EPDF$$P50$$Gproquest$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.proquest.com/docview/1449331977?pq-origsite=primo$$EHTML$$P50$$Gproquest$$Hfree_for_read</linktohtml><link.rule.ids>230,314,727,780,784,885,25753,27924,27925,37012,44590,53791,53793,75126</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/24244271$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><contributor>Menendez, Pablo</contributor><creatorcontrib>Conforti, Antonella</creatorcontrib><creatorcontrib>Biagini, Simone</creatorcontrib><creatorcontrib>Del Bufalo, Francesca</creatorcontrib><creatorcontrib>Sirleto, Pietro</creatorcontrib><creatorcontrib>Angioni, Adriano</creatorcontrib><creatorcontrib>Starc, Nadia</creatorcontrib><creatorcontrib>Li Pira, Giuseppina</creatorcontrib><creatorcontrib>Moretta, Francesca</creatorcontrib><creatorcontrib>Proia, Alessandra</creatorcontrib><creatorcontrib>Contoli, Benedetta</creatorcontrib><creatorcontrib>Genovese, Silvia</creatorcontrib><creatorcontrib>Ciardi, Claudia</creatorcontrib><creatorcontrib>Avanzini, Maria Antonietta</creatorcontrib><creatorcontrib>Rosti, Vittorio</creatorcontrib><creatorcontrib>Lo-Coco, Francesco</creatorcontrib><creatorcontrib>Locatelli, Franco</creatorcontrib><creatorcontrib>Bernardo, Maria Ester</creatorcontrib><title>Biological, functional and genetic characterization of bone marrow-derived mesenchymal stromal cells from pediatric patients affected by acute lymphoblastic leukemia</title><title>PloS one</title><addtitle>PLoS One</addtitle><description>Alterations in hematopoietic microenvironment of acute lymphoblastic leukemia patients have been claimed to occur, but little is known about the components of marrow stroma in these patients. In this study, we characterized mesenchymal stromal cells (MSCs) isolated from bone marrow (BM) of 45 pediatric patients with acute lymphoblastic leukemia (ALL-MSCs) at diagnosis (day+0) and during chemotherapy treatment (days: +15; +33; +78), the time points being chosen according to the schedule of BM aspirates required by the AIEOP-BFM ALL 2009 treatment protocol. Morphology, proliferative capacity, immunophenotype, differentiation potential, immunomodulatory properties and ability to support long-term hematopoiesis of ALL-MSCs were analysed and compared with those from 41 healthy donors (HD-MSCs). ALL-MSCs were also genetically characterized through array-CGH, conventional karyotyping and FISH analysis. Moreover, we compared ALL-MSCs generated at day+0 with those isolated during chemotherapy. Morphology, immunophenotype, differentiation potential and in vitro life-span did not differ between ALL-MSCs and HD-MSCs. ALL-MSCs showed significantly lower proliferative capacity (p&lt;0.001) and ability to support in vitro hematopoiesis (p = 0.04) as compared with HD-MSCs, while they had similar capacity to inhibit in vitro mitogen-induced T-cell proliferation (p = N.S.). ALL-MSCs showed neither the typical translocations carried by the leukemic clone (when present), nor other genetic abnormalities acquired during ex vivo culture. Our findings indicate that ALL-MSCs display reduced ability to proliferate and to support long-term hematopoiesis in vitro. ALL-MSCs isolated at diagnosis do not differ from those obtained during treatment.</description><subject>Abnormalities</subject><subject>Acute lymphoblastic leukemia</subject><subject>Acute lymphocytic leukemia</subject><subject>Adolescent</subject><subject>Analysis</subject><subject>Anemia</subject><subject>Antineoplastic Combined Chemotherapy Protocols - administration &amp; dosage</subject><subject>Blood diseases</subject><subject>Bone marrow</subject><subject>Bone Marrow Cells - metabolism</subject><subject>Bone Marrow Cells - pathology</subject><subject>Cancer</subject><subject>Cell culture</subject><subject>Cell Differentiation - drug effects</subject><subject>Cell proliferation</subject><subject>Cells, Cultured</subject><subject>Chemotherapy</subject><subject>Child</subject><subject>Child health</subject><subject>Child, Preschool</subject><subject>Children &amp; youth</subject><subject>Chromosomes</subject><subject>Cytogenetics</subject><subject>Diagnosis</subject><subject>Differentiation</subject><subject>Disease</subject><subject>Genetic abnormalities</subject><subject>Genetic aspects</subject><subject>Genetics</subject><subject>Hematology</subject><subject>Hematopoiesis</subject><subject>Hematopoiesis - drug effects</subject><subject>Hospitals</subject><subject>Humans</subject><subject>Immunomodulation</subject><subject>Infant</subject><subject>Laboratories</subject><subject>Leukemia</subject><subject>Lymphatic leukemia</subject><subject>Lymphocytes T</subject><subject>Male</subject><subject>Medical research</subject><subject>Mesenchymal stem cells</subject><subject>Mesenchymal Stromal Cells - metabolism</subject><subject>Mesenchymal Stromal Cells - pathology</subject><subject>Mesenchyme</subject><subject>Morphology</subject><subject>Oncology</subject><subject>Patients</subject><subject>Pediatrics</subject><subject>Precursor Cell Lymphoblastic Leukemia-Lymphoma - drug therapy</subject><subject>Precursor Cell Lymphoblastic Leukemia-Lymphoma - metabolism</subject><subject>Precursor Cell Lymphoblastic Leukemia-Lymphoma - pathology</subject><subject>Stem cells</subject><subject>Stromal cells</subject><subject>T cells</subject><subject>Time Factors</subject><subject>Translocation</subject><issn>1932-6203</issn><issn>1932-6203</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2013</creationdate><recordtype>article</recordtype><sourceid>PIMPY</sourceid><sourceid>DOA</sourceid><recordid>eNqNk22L1DAQx4so3nn6DUQDgiC4a9qkafJGOA8fFg4OfHobpul0N2fa7CXt6fp9_J6m7t6xCwrSF2lnfvOf4Z9Olj3O6TxnVf7q0o-hBzdf-x7nlFZCSXUnO84VK2aioOzu3vtR9iDGS0pLJoW4nx0VvOC8qPLj7Ncb651fWgPuJWnH3gzWJ1UCfUOW2ONgDTErCGAGDPYnTGniW1KnrqSDEPz3WZMy19iQDiP2ZrXpUn0cgp9Og85F0qYPssbGwhCS4DrJYD9EAm2LSbgh9YaAGQckbtOtV752EKfODsdv2Fl4mN1rwUV8tDtPsi_v3n4--zA7v3i_ODs9n5mqlMNMKmyFagpQEkxSNrIpVSVqplApIyUFIShQUyuV09YwTG7QIk9RXpVtbdhJ9nSru3Y-6p3DUeecK8ZyVVWJWGyJxsOlXgebPNhoD1b_Cfiw1BDS6A61AcW5NKaknPEKBTAQOaU1CBRClTJpvd51G-sOG5MsCeAORA8zvV3ppb_WTBZUVGUSeLYTCP5qxDj8Y-QdtYQ0le1bn8RMZ6PRp7ySTBVcTtT8L1R6mnQBJt12a1P8oODFQUFiBvwxLGGMUS8-ffx_9uLrIft8j10huGEVvRunPy8egnwLmuBjDNjeOpdTPa3IjRt6WhG9W5FU9mTf9duim51gvwHt1hDB</recordid><startdate>20131107</startdate><enddate>20131107</enddate><creator>Conforti, Antonella</creator><creator>Biagini, Simone</creator><creator>Del Bufalo, Francesca</creator><creator>Sirleto, Pietro</creator><creator>Angioni, Adriano</creator><creator>Starc, Nadia</creator><creator>Li Pira, Giuseppina</creator><creator>Moretta, Francesca</creator><creator>Proia, Alessandra</creator><creator>Contoli, Benedetta</creator><creator>Genovese, Silvia</creator><creator>Ciardi, Claudia</creator><creator>Avanzini, Maria Antonietta</creator><creator>Rosti, Vittorio</creator><creator>Lo-Coco, Francesco</creator><creator>Locatelli, Franco</creator><creator>Bernardo, Maria Ester</creator><general>Public Library of Science</general><general>Public Library of Science (PLoS)</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>IOV</scope><scope>ISR</scope><scope>3V.</scope><scope>7QG</scope><scope>7QL</scope><scope>7QO</scope><scope>7RV</scope><scope>7SN</scope><scope>7SS</scope><scope>7T5</scope><scope>7TG</scope><scope>7TM</scope><scope>7U9</scope><scope>7X2</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8AO</scope><scope>8C1</scope><scope>8FD</scope><scope>8FE</scope><scope>8FG</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABJCF</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>ARAPS</scope><scope>ATCPS</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BGLVJ</scope><scope>BHPHI</scope><scope>C1K</scope><scope>CCPQU</scope><scope>D1I</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>H94</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>KB.</scope><scope>KB0</scope><scope>KL.</scope><scope>L6V</scope><scope>LK8</scope><scope>M0K</scope><scope>M0S</scope><scope>M1P</scope><scope>M7N</scope><scope>M7P</scope><scope>M7S</scope><scope>NAPCQ</scope><scope>P5Z</scope><scope>P62</scope><scope>P64</scope><scope>PATMY</scope><scope>PDBOC</scope><scope>PIMPY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>PTHSS</scope><scope>PYCSY</scope><scope>RC3</scope><scope>5PM</scope><scope>DOA</scope></search><sort><creationdate>20131107</creationdate><title>Biological, functional and genetic characterization of bone marrow-derived mesenchymal stromal cells from pediatric patients affected by acute lymphoblastic leukemia</title><author>Conforti, Antonella ; Biagini, Simone ; Del Bufalo, Francesca ; Sirleto, Pietro ; Angioni, Adriano ; Starc, Nadia ; Li Pira, Giuseppina ; Moretta, Francesca ; Proia, Alessandra ; Contoli, Benedetta ; Genovese, Silvia ; Ciardi, Claudia ; Avanzini, Maria Antonietta ; Rosti, Vittorio ; Lo-Coco, Francesco ; Locatelli, Franco ; Bernardo, Maria Ester</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c758t-89ef69d2a98acfecc8d5976b39e99c880a660a0cb9910fc3e866021a66475fbc3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2013</creationdate><topic>Abnormalities</topic><topic>Acute lymphoblastic leukemia</topic><topic>Acute lymphocytic leukemia</topic><topic>Adolescent</topic><topic>Analysis</topic><topic>Anemia</topic><topic>Antineoplastic Combined Chemotherapy Protocols - administration &amp; dosage</topic><topic>Blood diseases</topic><topic>Bone marrow</topic><topic>Bone Marrow Cells - metabolism</topic><topic>Bone Marrow Cells - pathology</topic><topic>Cancer</topic><topic>Cell culture</topic><topic>Cell Differentiation - drug effects</topic><topic>Cell proliferation</topic><topic>Cells, Cultured</topic><topic>Chemotherapy</topic><topic>Child</topic><topic>Child health</topic><topic>Child, Preschool</topic><topic>Children &amp; youth</topic><topic>Chromosomes</topic><topic>Cytogenetics</topic><topic>Diagnosis</topic><topic>Differentiation</topic><topic>Disease</topic><topic>Genetic abnormalities</topic><topic>Genetic aspects</topic><topic>Genetics</topic><topic>Hematology</topic><topic>Hematopoiesis</topic><topic>Hematopoiesis - drug effects</topic><topic>Hospitals</topic><topic>Humans</topic><topic>Immunomodulation</topic><topic>Infant</topic><topic>Laboratories</topic><topic>Leukemia</topic><topic>Lymphatic leukemia</topic><topic>Lymphocytes T</topic><topic>Male</topic><topic>Medical research</topic><topic>Mesenchymal stem cells</topic><topic>Mesenchymal Stromal Cells - metabolism</topic><topic>Mesenchymal Stromal Cells - pathology</topic><topic>Mesenchyme</topic><topic>Morphology</topic><topic>Oncology</topic><topic>Patients</topic><topic>Pediatrics</topic><topic>Precursor Cell Lymphoblastic Leukemia-Lymphoma - drug therapy</topic><topic>Precursor Cell Lymphoblastic Leukemia-Lymphoma - metabolism</topic><topic>Precursor Cell Lymphoblastic Leukemia-Lymphoma - pathology</topic><topic>Stem cells</topic><topic>Stromal cells</topic><topic>T cells</topic><topic>Time Factors</topic><topic>Translocation</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Conforti, Antonella</creatorcontrib><creatorcontrib>Biagini, Simone</creatorcontrib><creatorcontrib>Del Bufalo, Francesca</creatorcontrib><creatorcontrib>Sirleto, Pietro</creatorcontrib><creatorcontrib>Angioni, Adriano</creatorcontrib><creatorcontrib>Starc, Nadia</creatorcontrib><creatorcontrib>Li Pira, Giuseppina</creatorcontrib><creatorcontrib>Moretta, Francesca</creatorcontrib><creatorcontrib>Proia, Alessandra</creatorcontrib><creatorcontrib>Contoli, Benedetta</creatorcontrib><creatorcontrib>Genovese, Silvia</creatorcontrib><creatorcontrib>Ciardi, Claudia</creatorcontrib><creatorcontrib>Avanzini, Maria Antonietta</creatorcontrib><creatorcontrib>Rosti, Vittorio</creatorcontrib><creatorcontrib>Lo-Coco, Francesco</creatorcontrib><creatorcontrib>Locatelli, Franco</creatorcontrib><creatorcontrib>Bernardo, Maria Ester</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Gale_Opposing Viewpoints In Context</collection><collection>Gale In Context: Science</collection><collection>ProQuest Central (Corporate)</collection><collection>Animal Behavior Abstracts</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Biotechnology Research Abstracts</collection><collection>Nursing &amp; Allied Health Database</collection><collection>Ecology Abstracts</collection><collection>Entomology Abstracts (Full archive)</collection><collection>Immunology Abstracts</collection><collection>Meteorological &amp; Geoastrophysical Abstracts</collection><collection>Nucleic Acids Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>Agricultural Science Collection</collection><collection>ProQuest - Health &amp; Medical Complete保健、医学与药学数据库</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Medical Database (Alumni Edition)</collection><collection>ProQuest Pharma Collection</collection><collection>Public Health Database</collection><collection>Technology Research Database</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Technology Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>Materials Science &amp; Engineering Collection</collection><collection>ProQuest Central (Alumni)</collection><collection>ProQuest Central</collection><collection>Advanced Technologies &amp; Aerospace Collection</collection><collection>ProQuest Agricultural &amp; Environmental Science</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>AUTh Library subscriptions: ProQuest Central</collection><collection>Technology Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ProQuest One Community College</collection><collection>ProQuest Materials Science Collection</collection><collection>ProQuest Central Korea</collection><collection>Engineering Research Database</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health &amp; Medical Complete (Alumni)</collection><collection>Materials Science Database</collection><collection>Nursing &amp; Allied Health Database (Alumni Edition)</collection><collection>Meteorological &amp; Geoastrophysical Abstracts - Academic</collection><collection>ProQuest Engineering Collection</collection><collection>ProQuest Biological Science Collection</collection><collection>Agriculture Science Database</collection><collection>Health &amp; Medical Collection (Alumni Edition)</collection><collection>PML(ProQuest Medical Library)</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biological Science Database</collection><collection>Engineering Database</collection><collection>Nursing &amp; Allied Health Premium</collection><collection>Advanced Technologies &amp; Aerospace Database</collection><collection>ProQuest Advanced Technologies &amp; Aerospace Collection</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Environmental Science Database</collection><collection>Materials Science Collection</collection><collection>Publicly Available Content Database</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>Engineering Collection</collection><collection>Environmental Science Collection</collection><collection>Genetics Abstracts</collection><collection>PubMed Central (Full Participant titles)</collection><collection>DOAJ Directory of Open Access Journals</collection><jtitle>PloS one</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Conforti, Antonella</au><au>Biagini, Simone</au><au>Del Bufalo, Francesca</au><au>Sirleto, Pietro</au><au>Angioni, Adriano</au><au>Starc, Nadia</au><au>Li Pira, Giuseppina</au><au>Moretta, Francesca</au><au>Proia, Alessandra</au><au>Contoli, Benedetta</au><au>Genovese, Silvia</au><au>Ciardi, Claudia</au><au>Avanzini, Maria Antonietta</au><au>Rosti, Vittorio</au><au>Lo-Coco, Francesco</au><au>Locatelli, Franco</au><au>Bernardo, Maria Ester</au><au>Menendez, Pablo</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Biological, functional and genetic characterization of bone marrow-derived mesenchymal stromal cells from pediatric patients affected by acute lymphoblastic leukemia</atitle><jtitle>PloS one</jtitle><addtitle>PLoS One</addtitle><date>2013-11-07</date><risdate>2013</risdate><volume>8</volume><issue>11</issue><spage>e76989</spage><pages>e76989-</pages><issn>1932-6203</issn><eissn>1932-6203</eissn><abstract>Alterations in hematopoietic microenvironment of acute lymphoblastic leukemia patients have been claimed to occur, but little is known about the components of marrow stroma in these patients. In this study, we characterized mesenchymal stromal cells (MSCs) isolated from bone marrow (BM) of 45 pediatric patients with acute lymphoblastic leukemia (ALL-MSCs) at diagnosis (day+0) and during chemotherapy treatment (days: +15; +33; +78), the time points being chosen according to the schedule of BM aspirates required by the AIEOP-BFM ALL 2009 treatment protocol. Morphology, proliferative capacity, immunophenotype, differentiation potential, immunomodulatory properties and ability to support long-term hematopoiesis of ALL-MSCs were analysed and compared with those from 41 healthy donors (HD-MSCs). ALL-MSCs were also genetically characterized through array-CGH, conventional karyotyping and FISH analysis. Moreover, we compared ALL-MSCs generated at day+0 with those isolated during chemotherapy. Morphology, immunophenotype, differentiation potential and in vitro life-span did not differ between ALL-MSCs and HD-MSCs. ALL-MSCs showed significantly lower proliferative capacity (p&lt;0.001) and ability to support in vitro hematopoiesis (p = 0.04) as compared with HD-MSCs, while they had similar capacity to inhibit in vitro mitogen-induced T-cell proliferation (p = N.S.). ALL-MSCs showed neither the typical translocations carried by the leukemic clone (when present), nor other genetic abnormalities acquired during ex vivo culture. Our findings indicate that ALL-MSCs display reduced ability to proliferate and to support long-term hematopoiesis in vitro. ALL-MSCs isolated at diagnosis do not differ from those obtained during treatment.</abstract><cop>United States</cop><pub>Public Library of Science</pub><pmid>24244271</pmid><doi>10.1371/journal.pone.0076989</doi><tpages>e76989</tpages><oa>free_for_read</oa></addata></record>
fulltext fulltext
identifier ISSN: 1932-6203
ispartof PloS one, 2013-11, Vol.8 (11), p.e76989
issn 1932-6203
1932-6203
language eng
recordid cdi_plos_journals_1449331977
source PubMed Central Free; Publicly Available Content Database
subjects Abnormalities
Acute lymphoblastic leukemia
Acute lymphocytic leukemia
Adolescent
Analysis
Anemia
Antineoplastic Combined Chemotherapy Protocols - administration & dosage
Blood diseases
Bone marrow
Bone Marrow Cells - metabolism
Bone Marrow Cells - pathology
Cancer
Cell culture
Cell Differentiation - drug effects
Cell proliferation
Cells, Cultured
Chemotherapy
Child
Child health
Child, Preschool
Children & youth
Chromosomes
Cytogenetics
Diagnosis
Differentiation
Disease
Genetic abnormalities
Genetic aspects
Genetics
Hematology
Hematopoiesis
Hematopoiesis - drug effects
Hospitals
Humans
Immunomodulation
Infant
Laboratories
Leukemia
Lymphatic leukemia
Lymphocytes T
Male
Medical research
Mesenchymal stem cells
Mesenchymal Stromal Cells - metabolism
Mesenchymal Stromal Cells - pathology
Mesenchyme
Morphology
Oncology
Patients
Pediatrics
Precursor Cell Lymphoblastic Leukemia-Lymphoma - drug therapy
Precursor Cell Lymphoblastic Leukemia-Lymphoma - metabolism
Precursor Cell Lymphoblastic Leukemia-Lymphoma - pathology
Stem cells
Stromal cells
T cells
Time Factors
Translocation
title Biological, functional and genetic characterization of bone marrow-derived mesenchymal stromal cells from pediatric patients affected by acute lymphoblastic leukemia
url http://sfxeu10.hosted.exlibrisgroup.com/loughborough?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-04T05%3A38%3A10IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-gale_plos_&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Biological,%20functional%20and%20genetic%20characterization%20of%20bone%20marrow-derived%20mesenchymal%20stromal%20cells%20from%20pediatric%20patients%20affected%20by%20acute%20lymphoblastic%20leukemia&rft.jtitle=PloS%20one&rft.au=Conforti,%20Antonella&rft.date=2013-11-07&rft.volume=8&rft.issue=11&rft.spage=e76989&rft.pages=e76989-&rft.issn=1932-6203&rft.eissn=1932-6203&rft_id=info:doi/10.1371/journal.pone.0076989&rft_dat=%3Cgale_plos_%3EA478392487%3C/gale_plos_%3E%3Cgrp_id%3Ecdi_FETCH-LOGICAL-c758t-89ef69d2a98acfecc8d5976b39e99c880a660a0cb9910fc3e866021a66475fbc3%3C/grp_id%3E%3Coa%3E%3C/oa%3E%3Curl%3E%3C/url%3E&rft_id=info:oai/&rft_pqid=1449331977&rft_id=info:pmid/24244271&rft_galeid=A478392487&rfr_iscdi=true