Enhancing the laccase production and laccase gene expression in the white-rot fungus Trametes velutina 5930 with great potential for biotechnological applications by different metal ions and aromatic compounds

• Published in: PloS one 2013-11, Vol.8 (11), p.e79307
• Main Authors: Yang, Yang, Wei, Fuxiang, Zhuo, Rui, Fan, Fangfang, Liu, Huahua, Zhang, Chen, Ma, Li, Jiang, Mulan, Zhang, Xiaoyu
• Format: Article
• Language: English
• Subjects: 5' Flanking Region; Acids; Agriculture; Aromatic compounds; Base Sequence; Basidiomycota - genetics; Basidiomycota - metabolism; Biology; Biotechnology; Chemical synthesis; Cinnamic acid; Cloning, Molecular; Copper; Enzymes; Extracellular Space - metabolism; Fungal Proteins - chemistry; Fungal Proteins - genetics; Fungi; Gallic acid; Gene expression; Gene Expression Regulation, Fungal - drug effects; Genes; Guaiacol; Industrial applications; Ions - metabolism; Ions - pharmacology; Iron; Laboratories; Laccase; Laccase - biosynthesis; Laccase - genetics; Life sciences; Lignin; Metal ions; Metals - metabolism; Metals - pharmacology; Molecular Sequence Data; Nature reserves; Oxidases; Pollutants; Promoter Regions, Genetic; Rot; Synergistic effect; Tannic acid; Technology application; Transcription; Transcription (Genetics); Transcription, Genetic; White rot fungi
• ISSN: 1932-6203; 1932-6203
• DOI: 10.1371/journal.pone.0079307

Laccase is useful for various biotechnological and industrial applications. The white-rot fungus Trametes velutina 5930 and its laccase, isolated from the Shennongjia Nature Reserve in China by our laboratory, has great potential for practical application in environmental biotechnology. However, the original level of laccase produced by Trametes velutina 5930 was relatively low in the absence of any inducer. Therefore, in order to enhance the laccase production by Trametes velutina 5930 and make better use of this fungus in the field of environmental biotechnology, the regulation of laccase production and laccase gene expression in Trametes velutina 5930 were investigated in this study. Different metal ions such as Cu(2+) and Fe(2+) could stimulate the laccase synthesis and laccase gene transcription in Trametes velutina 5930. Some aromatic compounds structurally related to lignin, such as tannic acid, syringic acid, cinnamic acid, gallic acid and guaiacol, could also enhance the level of laccase activity and laccase gene transcription. We also found that there existed a positive synergistic effect of aromatic compound and metal ion on the laccase production and laccase gene transcription in Trametes velutina 5930. Taken together, our study may contribute to the improvement of laccase productivity by Trametes velutina 5930.