Enhancing the laccase production and laccase gene expression in the white-rot fungus Trametes velutina 5930 with great potential for biotechnological applications by different metal ions and aromatic compounds

Laccase is useful for various biotechnological and industrial applications. The white-rot fungus Trametes velutina 5930 and its laccase, isolated from the Shennongjia Nature Reserve in China by our laboratory, has great potential for practical application in environmental biotechnology. However, the...

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Published in:PloS one 2013-11, Vol.8 (11), p.e79307
Main Authors: Yang, Yang, Wei, Fuxiang, Zhuo, Rui, Fan, Fangfang, Liu, Huahua, Zhang, Chen, Ma, Li, Jiang, Mulan, Zhang, Xiaoyu
Format: Article
Language:English
Subjects:
5' Flanking Region
Acids
Agriculture
Aromatic compounds
Base Sequence
Basidiomycota - genetics
Basidiomycota - metabolism
Biology
Biotechnology
Chemical synthesis
Cinnamic acid
Cloning, Molecular
Copper
Enzymes
Extracellular Space - metabolism
Fungal Proteins - chemistry
Fungal Proteins - genetics
Fungi
Gallic acid
Gene expression
Gene Expression Regulation, Fungal - drug effects
Genes
Guaiacol
Industrial applications
Ions - metabolism
Ions - pharmacology
Iron
Laboratories
Laccase
Laccase - biosynthesis
Laccase - genetics
Life sciences
Lignin
Metal ions
Metals - metabolism
Metals - pharmacology
Molecular Sequence Data
Nature reserves
Oxidases
Pollutants
Promoter Regions, Genetic
Rot
Synergistic effect
Tannic acid
Technology application
Transcription
Transcription (Genetics)
Transcription, Genetic
White rot fungi
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cited_by cdi_FETCH-LOGICAL-c692t-fcd478f23bdcbb4d50af608708364a9854ba48c908787f1b28f959c2e6ab21803
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container_issue 11
container_start_page e79307
container_title PloS one
container_volume 8
creator Yang, Yang
Wei, Fuxiang
Zhuo, Rui
Fan, Fangfang
Liu, Huahua
Zhang, Chen
Ma, Li
Jiang, Mulan
Zhang, Xiaoyu
description Laccase is useful for various biotechnological and industrial applications. The white-rot fungus Trametes velutina 5930 and its laccase, isolated from the Shennongjia Nature Reserve in China by our laboratory, has great potential for practical application in environmental biotechnology. However, the original level of laccase produced by Trametes velutina 5930 was relatively low in the absence of any inducer. Therefore, in order to enhance the laccase production by Trametes velutina 5930 and make better use of this fungus in the field of environmental biotechnology, the regulation of laccase production and laccase gene expression in Trametes velutina 5930 were investigated in this study. Different metal ions such as Cu(2+) and Fe(2+) could stimulate the laccase synthesis and laccase gene transcription in Trametes velutina 5930. Some aromatic compounds structurally related to lignin, such as tannic acid, syringic acid, cinnamic acid, gallic acid and guaiacol, could also enhance the level of laccase activity and laccase gene transcription. We also found that there existed a positive synergistic effect of aromatic compound and metal ion on the laccase production and laccase gene transcription in Trametes velutina 5930. Taken together, our study may contribute to the improvement of laccase productivity by Trametes velutina 5930.
doi_str_mv 10.1371/journal.pone.0079307
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The white-rot fungus Trametes velutina 5930 and its laccase, isolated from the Shennongjia Nature Reserve in China by our laboratory, has great potential for practical application in environmental biotechnology. However, the original level of laccase produced by Trametes velutina 5930 was relatively low in the absence of any inducer. Therefore, in order to enhance the laccase production by Trametes velutina 5930 and make better use of this fungus in the field of environmental biotechnology, the regulation of laccase production and laccase gene expression in Trametes velutina 5930 were investigated in this study. Different metal ions such as Cu(2+) and Fe(2+) could stimulate the laccase synthesis and laccase gene transcription in Trametes velutina 5930. Some aromatic compounds structurally related to lignin, such as tannic acid, syringic acid, cinnamic acid, gallic acid and guaiacol, could also enhance the level of laccase activity and laccase gene transcription. We also found that there existed a positive synergistic effect of aromatic compound and metal ion on the laccase production and laccase gene transcription in Trametes velutina 5930. 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genetics</topic><topic>Basidiomycota - metabolism</topic><topic>Biology</topic><topic>Biotechnology</topic><topic>Chemical synthesis</topic><topic>Cinnamic acid</topic><topic>Cloning, Molecular</topic><topic>Copper</topic><topic>Enzymes</topic><topic>Extracellular Space - metabolism</topic><topic>Fungal Proteins - chemistry</topic><topic>Fungal Proteins - genetics</topic><topic>Fungi</topic><topic>Gallic acid</topic><topic>Gene expression</topic><topic>Gene Expression Regulation, Fungal - drug effects</topic><topic>Genes</topic><topic>Guaiacol</topic><topic>Industrial applications</topic><topic>Ions - metabolism</topic><topic>Ions - pharmacology</topic><topic>Iron</topic><topic>Laboratories</topic><topic>Laccase</topic><topic>Laccase - biosynthesis</topic><topic>Laccase - genetics</topic><topic>Life sciences</topic><topic>Lignin</topic><topic>Metal ions</topic><topic>Metals - metabolism</topic><topic>Metals - pharmacology</topic><topic>Molecular Sequence Data</topic><topic>Nature reserves</topic><topic>Oxidases</topic><topic>Pollutants</topic><topic>Promoter Regions, Genetic</topic><topic>Rot</topic><topic>Synergistic effect</topic><topic>Tannic acid</topic><topic>Technology application</topic><topic>Transcription</topic><topic>Transcription (Genetics)</topic><topic>Transcription, Genetic</topic><topic>White rot fungi</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Yang, Yang</creatorcontrib><creatorcontrib>Wei, Fuxiang</creatorcontrib><creatorcontrib>Zhuo, Rui</creatorcontrib><creatorcontrib>Fan, Fangfang</creatorcontrib><creatorcontrib>Liu, Huahua</creatorcontrib><creatorcontrib>Zhang, Chen</creatorcontrib><creatorcontrib>Ma, Li</creatorcontrib><creatorcontrib>Jiang, Mulan</creatorcontrib><creatorcontrib>Zhang, Xiaoyu</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Gale in Context : Opposing Viewpoints</collection><collection>Gale in Context: Science</collection><collection>ProQuest Central (Corporate)</collection><collection>Animal Behavior Abstracts</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Biotechnology Research Abstracts</collection><collection>Nursing &amp; Allied Health Database (ProQuest)</collection><collection>Ecology Abstracts</collection><collection>Entomology Abstracts (Full archive)</collection><collection>Immunology Abstracts</collection><collection>Meteorological &amp; Geoastrophysical Abstracts</collection><collection>Nucleic Acids Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>Agricultural Science Collection</collection><collection>Health &amp; Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Medical Database (Alumni Edition)</collection><collection>ProQuest Pharma Collection</collection><collection>Public Health Database (Proquest)</collection><collection>Technology Research Database</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Technology Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>Materials Science &amp; Engineering Collection</collection><collection>ProQuest Central (Alumni)</collection><collection>ProQuest Central</collection><collection>Advanced Technologies &amp; Aerospace Collection</collection><collection>Agricultural &amp; Environmental Science Collection</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Technology Collection</collection><collection>Natural Science Collection</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ProQuest One Community College</collection><collection>ProQuest Materials Science Collection</collection><collection>ProQuest Central Korea</collection><collection>Engineering Research Database</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health &amp; Medical Complete (Alumni)</collection><collection>Materials Science Database</collection><collection>Nursing &amp; Allied Health Database (Alumni Edition)</collection><collection>Meteorological &amp; Geoastrophysical Abstracts - 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The white-rot fungus Trametes velutina 5930 and its laccase, isolated from the Shennongjia Nature Reserve in China by our laboratory, has great potential for practical application in environmental biotechnology. However, the original level of laccase produced by Trametes velutina 5930 was relatively low in the absence of any inducer. Therefore, in order to enhance the laccase production by Trametes velutina 5930 and make better use of this fungus in the field of environmental biotechnology, the regulation of laccase production and laccase gene expression in Trametes velutina 5930 were investigated in this study. Different metal ions such as Cu(2+) and Fe(2+) could stimulate the laccase synthesis and laccase gene transcription in Trametes velutina 5930. Some aromatic compounds structurally related to lignin, such as tannic acid, syringic acid, cinnamic acid, gallic acid and guaiacol, could also enhance the level of laccase activity and laccase gene transcription. We also found that there existed a positive synergistic effect of aromatic compound and metal ion on the laccase production and laccase gene transcription in Trametes velutina 5930. Taken together, our study may contribute to the improvement of laccase productivity by Trametes velutina 5930.</abstract><cop>United States</cop><pub>Public Library of Science</pub><pmid>24244475</pmid><doi>10.1371/journal.pone.0079307</doi><tpages>e79307</tpages><oa>free_for_read</oa></addata></record>
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source Publicly Available Content Database; PubMed Central
subjects 5' Flanking Region
Acids
Agriculture
Aromatic compounds
Base Sequence
Basidiomycota - genetics
Basidiomycota - metabolism
Biology
Biotechnology
Chemical synthesis
Cinnamic acid
Cloning, Molecular
Copper
Enzymes
Extracellular Space - metabolism
Fungal Proteins - chemistry
Fungal Proteins - genetics
Fungi
Gallic acid
Gene expression
Gene Expression Regulation, Fungal - drug effects
Genes
Guaiacol
Industrial applications
Ions - metabolism
Ions - pharmacology
Iron
Laboratories
Laccase
Laccase - biosynthesis
Laccase - genetics
Life sciences
Lignin
Metal ions
Metals - metabolism
Metals - pharmacology
Molecular Sequence Data
Nature reserves
Oxidases
Pollutants
Promoter Regions, Genetic
Rot
Synergistic effect
Tannic acid
Technology application
Transcription
Transcription (Genetics)
Transcription, Genetic
White rot fungi
title Enhancing the laccase production and laccase gene expression in the white-rot fungus Trametes velutina 5930 with great potential for biotechnological applications by different metal ions and aromatic compounds
url http://sfxeu10.hosted.exlibrisgroup.com/loughborough?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2024-12-18T17%3A55%3A53IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-gale_plos_&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Enhancing%20the%20laccase%20production%20and%20laccase%20gene%20expression%20in%20the%20white-rot%20fungus%20Trametes%20velutina%205930%20with%20great%20potential%20for%20biotechnological%20applications%20by%20different%20metal%20ions%20and%20aromatic%20compounds&rft.jtitle=PloS%20one&rft.au=Yang,%20Yang&rft.date=2013-11-11&rft.volume=8&rft.issue=11&rft.spage=e79307&rft.pages=e79307-&rft.issn=1932-6203&rft.eissn=1932-6203&rft_id=info:doi/10.1371/journal.pone.0079307&rft_dat=%3Cgale_plos_%3EA478321589%3C/gale_plos_%3E%3Cgrp_id%3Ecdi_FETCH-LOGICAL-c692t-fcd478f23bdcbb4d50af608708364a9854ba48c908787f1b28f959c2e6ab21803%3C/grp_id%3E%3Coa%3E%3C/oa%3E%3Curl%3E%3C/url%3E&rft_id=info:oai/&rft_pqid=1450016335&rft_id=info:pmid/24244475&rft_galeid=A478321589&rfr_iscdi=true