Diversity of both the cultivable protease-producing bacteria and bacterial extracellular proteases in the coastal sediments of King George Island, Antarctica

Protease-producing bacteria play a vital role in degrading sedimentary organic nitrogen. However, the diversity of these bacteria and their extracellular proteases in most regions remain unknown. In this paper, the diversity of the cultivable protease-producing bacteria and of bacterial extracellula...

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Bibliographic Details
Published in:PloS one 2013-11, Vol.8 (11), p.e79668-e79668
Main Authors: Zhou, Ming-Yang, Wang, Guang-Long, Li, Dan, Zhao, Dian-Li, Qin, Qi-Long, Chen, Xiu-Lan, Chen, Bo, Zhou, Bai-Cheng, Zhang, Xi-Ying, Zhang, Yu-Zhong
Format: Article
Language:English
Subjects:
Antarctic Regions
Bacteria
Bacteria - classification
Bacteria - cytology
Bacteria - growth & development
Bacteria - metabolism
Biodiversity
Biotechnology
Coastal sediments
Enzymes
Extracellular Space - enzymology
Flavobacterium
Genomics
Geologic Sediments - microbiology
Islands
Laboratories
Nitrogen
Organic nitrogen
Peptide Hydrolases - biosynthesis
Phylogenetics
Protease
Proteases
Proteinase
Proteinase inhibitors
Ribosomal DNA
Sediments
Sediments (Geology)
Serine
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Summary:Protease-producing bacteria play a vital role in degrading sedimentary organic nitrogen. However, the diversity of these bacteria and their extracellular proteases in most regions remain unknown. In this paper, the diversity of the cultivable protease-producing bacteria and of bacterial extracellular proteases in the sediments of Maxwell Bay, King George Island, Antarctica was investigated. The cultivable protease-producing bacteria reached 10(5) cells/g in all 8 sediment samples. The cultivated protease-producing bacteria were mainly affiliated with the phyla Actinobacteria, Firmicutes, Bacteroidetes, and Proteobacteria, and the predominant genera were Bacillus (22.9%), Flavobacterium (21.0%) and Lacinutrix (16.2%). Among these strains, Pseudoalteromonas and Flavobacteria showed relatively high protease production. Inhibitor analysis showed that nearly all the extracellular proteases from the bacteria were serine proteases or metalloproteases. These results begin to address the diversity of protease-producing bacteria and bacterial extracellular proteases in the sediments of the Antarctic Sea.
ISSN:1932-6203
1932-6203
DOI:10.1371/journal.pone.0079668