Identification of a new stromal cell type involved in the regulation of inflamed B cell follicles

Lymph node (LN) stromal cells provide survival signals and adhesive substrata to lymphocytes. During an immune response, B cell follicles enlarge, questioning how LN stromal cells manage these cellular demands. Herein, we used a murine fate mapping system to describe a new stromal cell type that res...

Full description

Saved in:
Bibliographic Details
Published in:PLoS biology 2013-10, Vol.11 (10), p.e1001672-e1001672
Main Authors: Mionnet, Cyrille, Mondor, Isabelle, Jorquera, Audrey, Loosveld, Marie, Maurizio, Julien, Arcangeli, Marie-Laure, Ruddle, Nancy H, Nowak, Jonathan, Aurrand-Lions, Michel, Luche, Hervé, Bajénoff, Marc
Format: Article
Language:English
Subjects:
Animals
B-Lymphocytes - pathology
Bone marrow
Boundaries
Chemokine CXCL13 - metabolism
Chemokines
Collagen
Dendritic Cells - pathology
Experiments
Fibroblasts - pathology
Immune system
Immunology
Inflammation - immunology
Inflammation - pathology
Life Sciences
Lymph Nodes - pathology
Lymphocyte Depletion
Lymphocytes
Lymphocytes - pathology
Lymphoma
Mice
Microscopy
Proteins
Receptors, Complement 3d - metabolism
Receptors, CXCR5 - deficiency
Receptors, CXCR5 - metabolism
Rodents
Stromal Cells - metabolism
Stromal Cells - pathology
T-Lymphocytes
Trespassing
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Lymph node (LN) stromal cells provide survival signals and adhesive substrata to lymphocytes. During an immune response, B cell follicles enlarge, questioning how LN stromal cells manage these cellular demands. Herein, we used a murine fate mapping system to describe a new stromal cell type that resides in the T cell zone of resting LNs. We demonstrated that upon inflammation, B cell follicles progressively trespassed into the adjacent T cell zone and surrounded and converted these stromal cells into CXCL13 secreting cells that in return delineated the new boundaries of the growing follicle. Acute B cell ablation in inflamed LNs abolished CXCL13 secretion in these cells, while LT-β deficiency in B cells drastically affected this conversion. Altogether, we reveal the existence of a dormant stromal cell subset that can be functionally awakened by B cells to delineate the transient boundaries of their expanding territories upon inflammation.
ISSN:1545-7885
1544-9173
1545-7885
DOI:10.1371/journal.pbio.1001672