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Rapid diagnosis of diarrhea caused by Shigella sonnei using dipsticks; comparison of rectal swabs, direct stool and stool culture

We evaluated a dipstick test for rapid detection of Shigella sonnei on bacterial colonies, directly on stools and from rectal swabs because in actual field situations, most pathologic specimens for diagnosis correspond to stool samples or rectal swabs. The test is based on the detection of S. sonnei...

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Published in:PloS one 2013-11, Vol.8 (11), p.e80267
Main Authors: Duran, Claudia, Nato, Faridabano, Dartevelle, Sylvie, Thi Phuong, Lan Nguyen, Taneja, Neelam, Ungeheuer, Marie Noëlle, Soza, Guillermo, Anderson, Leslie, Benadof, Dona, Zamorano, Agustín, Diep, Tai The, Nguyen, Truong Quang, Nguyen, Vu Hoang, Ottone, Catherine, Bégaud, Evelyne, Pahil, Sapna, Prado, Valeria, Sansonetti, Philippe, Germani, Yves
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cited_by cdi_FETCH-LOGICAL-c692t-b5a2d988209c5c60a6768e0e9c5f84a71e38aa647a402986fceda8eda5c6b91b3
cites cdi_FETCH-LOGICAL-c692t-b5a2d988209c5c60a6768e0e9c5f84a71e38aa647a402986fceda8eda5c6b91b3
container_end_page
container_issue 11
container_start_page e80267
container_title PloS one
container_volume 8
creator Duran, Claudia
Nato, Faridabano
Dartevelle, Sylvie
Thi Phuong, Lan Nguyen
Taneja, Neelam
Ungeheuer, Marie Noëlle
Soza, Guillermo
Anderson, Leslie
Benadof, Dona
Zamorano, Agustín
Diep, Tai The
Nguyen, Truong Quang
Nguyen, Vu Hoang
Ottone, Catherine
Bégaud, Evelyne
Pahil, Sapna
Prado, Valeria
Sansonetti, Philippe
Germani, Yves
description We evaluated a dipstick test for rapid detection of Shigella sonnei on bacterial colonies, directly on stools and from rectal swabs because in actual field situations, most pathologic specimens for diagnosis correspond to stool samples or rectal swabs. The test is based on the detection of S. sonnei lipopolysaccharide (LPS) O-side chains using phase I-specific monoclonal antibodies coupled to gold particles, and displayed on a one-step immunochromatographic dipstick. A concentration as low as 5 ng/ml of LPS was detected in distilled water and in reconstituted stools in 6 minutes. This is the optimal time for lecture to avoid errors of interpretation. In distilled water and in reconstituted stools, an unequivocal positive reaction was obtained with 4 x 10(6) CFU/ml of S. sonnei. The specificity was 100% when tested with a battery of Shigella and different unrelated strains. When tested on 342 rectal swabs in Chile, specificity (281/295) was 95.3% (95% CI: 92.9% - 97.7%) and sensitivity (47/47) was 100%. Stool cultures and the immunochromatographic test showed concordant results in 95.5 % of cases (328/342) in comparative studies. Positive and negative predictive values were 77% (95% CI: 65% - 86.5%) and 100% respectively. When tested on 219 stools in Chile, Vietnam, India and France, specificity (190/198) was 96% (95% CI 92%-98%) and sensitivity (21/21) was 100%. Stool cultures and the immunochromatographic test showed concordant results in 96.3 % of cases (211/219) in comparative studies. Positive and negative predictive values were 72.4% (95% CI 56.1%-88.6%) and 100 %, respectively. This one-step dipstick test performed well for diagnosis of S. sonnei both on stools and on rectal swabs. These data confirm a preliminary study done in Chile.
doi_str_mv 10.1371/journal.pone.0080267
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Allied Health Database (Alumni Edition)</collection><collection>Meteorological &amp; Geoastrophysical Abstracts - Academic</collection><collection>ProQuest Engineering Collection</collection><collection>ProQuest Biological Science Collection</collection><collection>Agriculture Science Database</collection><collection>Health &amp; Medical Collection (Alumni Edition)</collection><collection>PML(ProQuest Medical Library)</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>ProQuest Biological Science Journals</collection><collection>Engineering Database</collection><collection>Nursing &amp; Allied Health Premium</collection><collection>Advanced Technologies &amp; Aerospace Database</collection><collection>ProQuest Advanced Technologies &amp; Aerospace Collection</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Environmental Science Database</collection><collection>Materials Science Collection</collection><collection>Publicly Available Content (ProQuest)</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>Engineering Collection</collection><collection>Environmental Science Collection</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><collection>DOAJ Directory of Open Access Journals</collection><jtitle>PloS one</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Duran, Claudia</au><au>Nato, Faridabano</au><au>Dartevelle, Sylvie</au><au>Thi Phuong, Lan Nguyen</au><au>Taneja, Neelam</au><au>Ungeheuer, Marie Noëlle</au><au>Soza, Guillermo</au><au>Anderson, Leslie</au><au>Benadof, Dona</au><au>Zamorano, Agustín</au><au>Diep, Tai The</au><au>Nguyen, Truong Quang</au><au>Nguyen, Vu Hoang</au><au>Ottone, Catherine</au><au>Bégaud, Evelyne</au><au>Pahil, Sapna</au><au>Prado, Valeria</au><au>Sansonetti, Philippe</au><au>Germani, Yves</au><au>Foligne, Benoit</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Rapid diagnosis of diarrhea caused by Shigella sonnei using dipsticks; comparison of rectal swabs, direct stool and stool culture</atitle><jtitle>PloS one</jtitle><addtitle>PLoS One</addtitle><date>2013-11-22</date><risdate>2013</risdate><volume>8</volume><issue>11</issue><spage>e80267</spage><pages>e80267-</pages><issn>1932-6203</issn><eissn>1932-6203</eissn><abstract>We evaluated a dipstick test for rapid detection of Shigella sonnei on bacterial colonies, directly on stools and from rectal swabs because in actual field situations, most pathologic specimens for diagnosis correspond to stool samples or rectal swabs. The test is based on the detection of S. sonnei lipopolysaccharide (LPS) O-side chains using phase I-specific monoclonal antibodies coupled to gold particles, and displayed on a one-step immunochromatographic dipstick. A concentration as low as 5 ng/ml of LPS was detected in distilled water and in reconstituted stools in 6 minutes. This is the optimal time for lecture to avoid errors of interpretation. In distilled water and in reconstituted stools, an unequivocal positive reaction was obtained with 4 x 10(6) CFU/ml of S. sonnei. The specificity was 100% when tested with a battery of Shigella and different unrelated strains. When tested on 342 rectal swabs in Chile, specificity (281/295) was 95.3% (95% CI: 92.9% - 97.7%) and sensitivity (47/47) was 100%. Stool cultures and the immunochromatographic test showed concordant results in 95.5 % of cases (328/342) in comparative studies. Positive and negative predictive values were 77% (95% CI: 65% - 86.5%) and 100% respectively. When tested on 219 stools in Chile, Vietnam, India and France, specificity (190/198) was 96% (95% CI 92%-98%) and sensitivity (21/21) was 100%. Stool cultures and the immunochromatographic test showed concordant results in 96.3 % of cases (211/219) in comparative studies. Positive and negative predictive values were 72.4% (95% CI 56.1%-88.6%) and 100 %, respectively. This one-step dipstick test performed well for diagnosis of S. sonnei both on stools and on rectal swabs. These data confirm a preliminary study done in Chile.</abstract><cop>United States</cop><pub>Public Library of Science</pub><pmid>24278267</pmid><doi>10.1371/journal.pone.0080267</doi><tpages>e80267</tpages><oa>free_for_read</oa></addata></record>
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identifier ISSN: 1932-6203
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issn 1932-6203
1932-6203
language eng
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source PubMed Central(OpenAccess); Publicly Available Content (ProQuest)
subjects Antigens
Bacteria
Batteries
Colonies
Comparative analysis
Comparative studies
Diagnosis
Diarrhea
Diarrhea - diagnosis
Diarrhea - microbiology
Distilled water
E coli
Escherichia coli
Ethics
Feces - microbiology
Gold
Health education
Hospitals
Humans
Immunology
Industrialized nations
Lipopolysaccharides
Medical diagnosis
Mitogens
Monoclonal antibodies
Pathogens
Polymerase chain reaction
Rectum
Rectum - microbiology
Reproducibility of Results
Sensitivity
Sensitivity and Specificity
Shigella
Shigella sonnei - isolation & purification
Shigella sonnei - pathogenicity
Shigellosis
title Rapid diagnosis of diarrhea caused by Shigella sonnei using dipsticks; comparison of rectal swabs, direct stool and stool culture
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