Monocytes loaded with indocyanine green as active homing contrast agents permit optical differentiation of infectious and non-infectious inflammation

Distinguishing cutaneous infection from sterile inflammation is a diagnostic challenge and currently relies upon subjective interpretation of clinical parameters, microbiological data, and nonspecific imaging. Assessing characteristic variations in leukocytic infiltration may provide more specific i...

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Published in:PloS one 2013-11, Vol.8 (11), p.e81430-e81430
Main Authors: Christensen, Joani M, Brat, Gabriel A, Johnson, Kristine E, Chen, Yongping, Buretta, Kate J, Cooney, Damon S, Brandacher, Gerald, Lee, W P Andrew, Li, Xingde, Sacks, Justin M
Format: Article
Language:English
Subjects:
Angiography
Animals
Arthritis
Bacteria
Biomedical engineering
Biopsy
Blood
Cancer
Cell Line
Contrast agents
Contrast Media
Diagnostic systems
Fluorescence
Homing
Immunotherapy
Indocyanine Green - metabolism
Infections
Infectious diseases
Infiltration
Inflammation
Inflammation - diagnosis
Injection
Inoculation
Laboratories
Medical imaging
Medicine
Mice
Monocyte chemoattractant protein 1
Monocytes
Monocytes - metabolism
Near infrared radiation
Pain
Plastic surgery
Quantum dots
Radiation
Stem cells
Streptococcus infections
Systematic review
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Li, Xingde
Sacks, Justin M
description Distinguishing cutaneous infection from sterile inflammation is a diagnostic challenge and currently relies upon subjective interpretation of clinical parameters, microbiological data, and nonspecific imaging. Assessing characteristic variations in leukocytic infiltration may provide more specific information. In this study, we demonstrate that homing of systemically administered monocytes tagged using indocyanine green (ICG), an FDA-approved near infrared dye, may be assessed non-invasively using clinically-applicable laser angiography systems to investigate cutaneous inflammatory processes. RAW 264.7 mouse monocytes co-incubated with ICG fluoresce brightly in the near infrared range. In vitro, the loaded cells retained the ability to chemotax toward monocyte chemotactic protein-1. Following intravascular injection of loaded cells into BALB/c mice with induced sterile inflammation (Complete Freund's Adjuvant inoculation) or infection (Group A Streptococcus inoculation) of the hind limb, non-invasive whole animal imaging revealed local fluorescence at the inoculation site. There was significantly higher fluorescence of the inoculation site in the infection model than in the inflammation model as early as 2 hours after injection (p
doi_str_mv 10.1371/journal.pone.0081430
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Assessing characteristic variations in leukocytic infiltration may provide more specific information. In this study, we demonstrate that homing of systemically administered monocytes tagged using indocyanine green (ICG), an FDA-approved near infrared dye, may be assessed non-invasively using clinically-applicable laser angiography systems to investigate cutaneous inflammatory processes. RAW 264.7 mouse monocytes co-incubated with ICG fluoresce brightly in the near infrared range. In vitro, the loaded cells retained the ability to chemotax toward monocyte chemotactic protein-1. Following intravascular injection of loaded cells into BALB/c mice with induced sterile inflammation (Complete Freund's Adjuvant inoculation) or infection (Group A Streptococcus inoculation) of the hind limb, non-invasive whole animal imaging revealed local fluorescence at the inoculation site. There was significantly higher fluorescence of the inoculation site in the infection model than in the inflammation model as early as 2 hours after injection (p&lt;0.05). Microscopic examination of bacterial inoculation site tissue revealed points of near infrared fluorescence, suggesting the presence of ICG-loaded cells. 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subjects Angiography
Animals
Arthritis
Bacteria
Biomedical engineering
Biopsy
Blood
Cancer
Cell Line
Contrast agents
Contrast Media
Diagnostic systems
Fluorescence
Homing
Immunotherapy
Indocyanine Green - metabolism
Infections
Infectious diseases
Infiltration
Inflammation
Inflammation - diagnosis
Injection
Inoculation
Laboratories
Medical imaging
Medicine
Mice
Monocyte chemoattractant protein 1
Monocytes
Monocytes - metabolism
Near infrared radiation
Pain
Plastic surgery
Quantum dots
Radiation
Stem cells
Streptococcus infections
Systematic review
title Monocytes loaded with indocyanine green as active homing contrast agents permit optical differentiation of infectious and non-infectious inflammation
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