Constitutive function of the Ikaros transcription factor in primary leukemia cells from pediatric newly diagnosed high-risk and relapsed B-precursor ALL patients

We examined the constitutive function of the Ikaros (IK) transcription factor in blast cells from pediatric B-precursor acute lymphoblastic leukemia (BPL) patients using multiple assay platforms and bioinformatics tools. We found no evidence of diminished IK expression or function for primary cells...

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Bibliographic Details
Published in:PloS one 2013-11, Vol.8 (11), p.e80732
Main Authors: Uckun, Fatih M, Ma, Hong, Ishkhanian, Rita, Arellano, Martha, Shahidzadeh, Anoush, Termuhlen, Amanda, Gaynon, Paul S, Qazi, Sanjive
Format: Article
Language:English
Subjects:
Acute lymphoblastic leukemia
Animals
Arrays
Bioinformatics
Blast cells
Blood diseases
Blotting, Western
Cancer
Child
Deoxyribonucleic acid
DNA
Electrophoretic Mobility Shift Assay
Exons - genetics
Female
Gene Deletion
Gene expression
Gene Expression Profiling
Gene Expression Regulation, Leukemic
Genes, Neoplasm
Genomics
Hospitals
Human subjects
Humans
Ikaros protein
Ikaros Transcription Factor - genetics
Ikaros Transcription Factor - metabolism
Inoculation
Laboratory animals
Leukemia
Localization
Lymphatic leukemia
Lymphocytes
Lymphocytes B
Medical diagnosis
Mice, Inbred NOD
Mice, SCID
Patients
Pediatrics
Philadelphia Chromosome
Precursor B-Cell Lymphoblastic Leukemia-Lymphoma - diagnosis
Precursor B-Cell Lymphoblastic Leukemia-Lymphoma - genetics
Precursors
Proteins
Real-Time Polymerase Chain Reaction
Recurrence
Risk
Risk Factors
RNA, Messenger - genetics
RNA, Messenger - metabolism
Tumor Cells, Cultured
Xenograft Model Antitumor Assays
Xenografts
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Summary:We examined the constitutive function of the Ikaros (IK) transcription factor in blast cells from pediatric B-precursor acute lymphoblastic leukemia (BPL) patients using multiple assay platforms and bioinformatics tools. We found no evidence of diminished IK expression or function for primary cells from high-risk BPL patients including a Philadelphia chromosome (Ph)(+) subset. Relapse clones as well as very aggressive in vivo clonogenic leukemic B-cell precursors isolated from spleens of xenografted NOD/SCID mice that developed overt leukemia after inoculation with primary leukemic cells of patients with BPL invariably and abundantly expressed intact IK protein. These results demonstrate that a lost or diminished IK function is not a characteristic feature of leukemic cells in Ph(+) or Ph(-) high-risk BPL.
ISSN:1932-6203
1932-6203
DOI:10.1371/journal.pone.0080732