The maize glossy13 gene, cloned via BSR-Seq and Seq-walking encodes a putative ABC transporter required for the normal accumulation of epicuticular waxes

Aerial plant surfaces are covered by epicuticular waxes that among other purposes serve to control water loss. Maize glossy mutants originally identified by their "glossy" phenotypes exhibit alterations in the accumulation of epicuticular waxes. By combining data from a BSR-Seq experiment...

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Bibliographic Details
Published in:PloS one 2013-12, Vol.8 (12), p.e82333
Main Authors: Li, Li, Li, Delin, Liu, Sanzhen, Ma, Xiaoli, Dietrich, Charles R, Hu, Heng-Cheng, Zhang, Gaisheng, Liu, Zhiyong, Zheng, Jun, Wang, Guoying, Schnable, Patrick S
Format: Article
Language:English
Subjects:
ABC transporter
Accumulation
Agriculture
Agronomy
Alleles
Arabidopsis
Arabidopsis thaliana
ATP-Binding Cassette Transporters - classification
ATP-Binding Cassette Transporters - genetics
ATP-Binding Cassette Transporters - metabolism
Barley
Chromosome Mapping
Chromosomes, Plant
Climate change
Cloning
Cloning, Molecular
Codons
Corn
Crop science
Cuticular wax
Fatty acids
Gene expression
Gene Expression Regulation, Plant
Gene Order
Genetics
Genomics
Leaves
Metabolites
Mutants
Mutation
Phenotype
Phylogeny
Plant Proteins - genetics
Plant Proteins - metabolism
Proteins
Quantitative Trait, Heritable
Reproducibility of Results
Secondary metabolites
Seedlings
Transcription factors
Transport
Walking
Water loss
Waxes
Waxes - metabolism
Zea mays - genetics
Zea mays - metabolism
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Summary:Aerial plant surfaces are covered by epicuticular waxes that among other purposes serve to control water loss. Maize glossy mutants originally identified by their "glossy" phenotypes exhibit alterations in the accumulation of epicuticular waxes. By combining data from a BSR-Seq experiment and the newly developed Seq-Walking technology, GRMZM2G118243 was identified as a strong candidate for being the glossy13 gene. The finding that multiple EMS-induced alleles contain premature stop codons in GRMZM2G118243, and the one knockout allele of gl13, validates the hypothesis that gene GRMZM2G118243 is gl13. Consistent with this, GRMZM2G118243 is an ortholog of AtABCG32 (Arabidopsis thaliana), HvABCG31 (barley) and OsABCG31 (rice), which encode ABCG subfamily transporters involved in the trans-membrane transport of various secondary metabolites. We therefore hypothesize that gl13 is involved in the transport of epicuticular waxes onto the surfaces of seedling leaves.
ISSN:1932-6203
1932-6203
DOI:10.1371/journal.pone.0082333