Natural killer cell-dependent anti-fibrotic pathway in liver injury via Toll-like receptor-9

The toll-like receptor-9 (TLR9) agonist cytosine phosphate guanine (CpG), activates hepatic stellate cells (HSCs) and mediates fibrosis. We investigated the TLR9 effects on lymphocyte/HSCs interactions. Liver fibrosis was induced in wild-type (WT) mice by intra-peritoneal carbon-tetrachloride (CCl4)...

Full description

Saved in:
Bibliographic Details
Published in:PloS one 2013-12, Vol.8 (12), p.e82571
Main Authors: Abu-Tair, Lina, Axelrod, Jonathan H, Doron, Sarit, Ovadya, Yossi, Krizhanovsky, Valery, Galun, Eithan, Amer, Johnny, Safadi, Rifaat
Format: Article
Language:English
Subjects:
Adjuvants, Immunologic - pharmacology
Adoptive Transfer
Animals
Apoptosis
Carbon tetrachloride
Carbon Tetrachloride Poisoning - genetics
Carbon Tetrachloride Poisoning - immunology
Carbon Tetrachloride Poisoning - pathology
CCL4 protein
CD8 antigen
CD8-Positive T-Lymphocytes - immunology
CD8-Positive T-Lymphocytes - pathology
Cell activation
Cell Communication - drug effects
Cell Communication - genetics
Cell Communication - immunology
Cell proliferation
CpG islands
Cytokines
Cytosine
Deoxyribonucleic acid
DNA
Drug dosages
Fibrosis
Gastroenterology
Gene therapy
Guanine
Hepatic Stellate Cells - immunology
Hepatic Stellate Cells - pathology
Hepatitis
Hepatocytes
Hepatology
Inflammation
Killer cells
Killer Cells, Natural
Laboratory animals
Liver
Liver Cirrhosis - chemically induced
Liver Cirrhosis - genetics
Liver Cirrhosis - immunology
Liver Cirrhosis - pathology
Liver diseases
Lymphocytes
Medicine
Mice
Mice, Knockout
Natural killer cells
Oligodeoxyribonucleotides - pharmacology
Peritoneum
Phosphates
Pyrimidines
Rodents
Senescence
Serum levels
Stellate cells
TLR9 protein
Toll-Like Receptor 9 - genetics
Toll-Like Receptor 9 - immunology
Toll-like receptors
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:The toll-like receptor-9 (TLR9) agonist cytosine phosphate guanine (CpG), activates hepatic stellate cells (HSCs) and mediates fibrosis. We investigated the TLR9 effects on lymphocyte/HSCs interactions. Liver fibrosis was induced in wild-type (WT) mice by intra-peritoneal carbon-tetrachloride (CCl4) induction for 6 weeks. Fibrotic groups were intravenously treated by a vehicle versus CpG along last 2 weeks. Compared to vehicle-treated fibrotic WT, the in-vivo CpG-treatment significantly attenuated hepatic fibrosis and inflammation, associated with decreased CD8 and increased NK liver cells. In-vitro, co-cultures with vehicle-treated fibrotic NK cells increased HSCs proliferation (P
ISSN:1932-6203
1932-6203
DOI:10.1371/journal.pone.0082571