An amino acid substitution (L925V) associated with resistance to pyrethroids in Varroa destructor

The Varroa mite, Varroa destructor, is an important pest of honeybees and has played a prominent role in the decline in bee colony numbers over recent years. Although pyrethroids such as tau-fluvalinate and flumethrin can be highly effective in removing the mites from hives, their intensive use has...

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Published in:PloS one 2013-12, Vol.8 (12), p.e82941
Main Authors: González-Cabrera, Joel, Davies, T G Emyr, Field, Linda M, Kennedy, Peter J, Williamson, Martin S
Format: Article
Language:English
Subjects:
Acids
Amino Acid Substitution
Amino acids
Animals
Apis mellifera
Bees
Binding sites
Biochemistry
Chemistry
Diagnostic systems
Drug Resistance
European honeybee
Fluvalinate
Hot spots
Insecticides
Insecticides - pharmacology
Laboratories
Management science
Mites
Molecular biology
Mutation
Polymerase Chain Reaction
Pyrethrins - pharmacology
Pyrethroids
Sodium
Sodium channels (voltage-gated)
Studies
Substitutes
Trialeurodes vaporariorum
Varroa destructor
Varroidae - drug effects
Varroidae - genetics
Varroidae - metabolism
Voltage-Gated Sodium Channels - chemistry
Voltage-Gated Sodium Channels - genetics
Voltage-Gated Sodium Channels - metabolism
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cited_by cdi_FETCH-LOGICAL-c593t-911a3fb23cc2072878fc7ffb65278311a44286402be985df44abd8e80ac210b03
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Davies, T G Emyr
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description The Varroa mite, Varroa destructor, is an important pest of honeybees and has played a prominent role in the decline in bee colony numbers over recent years. Although pyrethroids such as tau-fluvalinate and flumethrin can be highly effective in removing the mites from hives, their intensive use has led to many reports of resistance. To investigate the mechanism of resistance in UK Varroa samples, the transmembrane domain regions of the V. destructor voltage-gated sodium channel (the main target site for pyrethroids) were PCR amplified and sequenced from pyrethroid treated/untreated mites collected at several locations in Central/Southern England. A novel amino acid substitution, L925V, was identified that maps to a known hot spot for resistance within the domain IIS5 helix of the channel protein; a region that has also been proposed to form part of the pyrethroid binding site. Using a high throughput diagnostic assay capable of detecting the mutation in individual mites, the L925V substitution was found to correlate well with resistance, being present in all mites that had survived tau-fluvalinate treatment but in only 8 % of control, untreated samples. The potential for using this assay to detect and manage resistance in Varroa-infected hives is discussed.
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Using a high throughput diagnostic assay capable of detecting the mutation in individual mites, the L925V substitution was found to correlate well with resistance, being present in all mites that had survived tau-fluvalinate treatment but in only 8 % of control, untreated samples. 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subjects Acids
Amino Acid Substitution
Amino acids
Animals
Apis mellifera
Bees
Binding sites
Biochemistry
Chemistry
Diagnostic systems
Drug Resistance
European honeybee
Fluvalinate
Hot spots
Insecticides
Insecticides - pharmacology
Laboratories
Management science
Mites
Molecular biology
Mutation
Polymerase Chain Reaction
Pyrethrins - pharmacology
Pyrethroids
Sodium
Sodium channels (voltage-gated)
Studies
Substitutes
Trialeurodes vaporariorum
Varroa destructor
Varroidae - drug effects
Varroidae - genetics
Varroidae - metabolism
Voltage-Gated Sodium Channels - chemistry
Voltage-Gated Sodium Channels - genetics
Voltage-Gated Sodium Channels - metabolism
title An amino acid substitution (L925V) associated with resistance to pyrethroids in Varroa destructor
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