Exposure to biomass smoke extract enhances fibronectin release from fibroblasts

COPD induced following biomass smoke exposure has been reported to be associated with a more fibrotic phenotype than cigarette smoke induced COPD. This study aimed to investigate if biomass smoke induced extracellular matrix (ECM) protein production from primary human lung fibroblasts in vitro. Prim...

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Bibliographic Details
Published in:PloS one 2013-12, Vol.8 (12), p.e83938
Main Authors: Krimmer, David, Ichimaru, Yukikazu, Burgess, Janette, Black, Judith, Oliver, Brian
Format: Article
Language:English
Subjects:
Adult
Aged
Airway Remodeling - drug effects
Antibiotics
Biomass
Cell growth
Chronic obstructive pulmonary disease
Cigarette smoke
Cigarettes
Cytokines
Deposition
Discipline
Disease
Exposure
Extracellular matrix
Extracellular signal-regulated kinase
Extracellular Signal-Regulated MAP Kinases - antagonists & inhibitors
Female
Fibroblasts
Fibroblasts - drug effects
Fibroblasts - secretion
Fibronectin
Fibronectins - secretion
Fibrosis
Forced Expiratory Volume - drug effects
Gene expression
Health services
Humans
Interleukin 8
Interleukin-8 - secretion
Intracellular signalling
Leukocytes (neutrophilic)
Lungs
Male
Medical research
Middle Aged
Neutrophils
Perlecan
Pharmacology
Phosphorylation
Phosphorylation - drug effects
Protein Kinase Inhibitors - pharmacology
Proteins
Rodents
Smoke
Smoke - adverse effects
Smoking
Smooth muscle
Tobacco Products - analysis
Tobacco smoke
VOCs
Volatile organic compounds
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Summary:COPD induced following biomass smoke exposure has been reported to be associated with a more fibrotic phenotype than cigarette smoke induced COPD. This study aimed to investigate if biomass smoke induced extracellular matrix (ECM) protein production from primary human lung fibroblasts in vitro. Primary human lung fibroblasts (n=5-10) were stimulated in vitro for up to 72 hours with increasing concentrations of biomass smoke extract (BME) or cigarette smoke extract (CSE) prior to being assessed for deposition of ECM proteins, cytokine release, and activation of intracellular signalling molecules. Deposition of the ECM proteins perlecan and fibronectin was upregulated by both CSE (p
ISSN:1932-6203
1932-6203
DOI:10.1371/journal.pone.0083938