Relationship between vitreous levels of matrix metalloproteinases and vascular endothelial growth factor in proliferative diabetic retinopathy

To investigate which matrix metalloproteinases (MMPs) are more likely to be involved in the angiogenic process in proliferative diabetic retinopathy (PDR), we measured the levels of MMPs in the vitreous fluid from patients with PDR and controls and correlated these levels with the levels of vascular...

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Bibliographic Details
Published in:PloS one 2013-12, Vol.8 (12), p.e85857-e85857
Main Authors: Abu El-Asrar, Ahmed M, Mohammad, Ghulam, Nawaz, Mohd Imtiaz, Siddiquei, Mohammad Mairaj, Van den Eynde, Kathleen, Mousa, Ahmed, De Hertogh, Gert, Opdenakker, Ghislain
Format: Article
Language:English
Subjects:
Analysis
Angiogenesis
Assaying
Cancer
Cell adhesion & migration
Collagenase 3
Consent
Correlation
Diabetes
Diabetes mellitus
Diabetic retinopathy
Diabetic Retinopathy - complications
Diabetic Retinopathy - enzymology
Diabetic Retinopathy - metabolism
Diabetic Retinopathy - pathology
Endothelial cells
Enzyme-linked immunosorbent assay
Enzymes
Gelatin
Gelatinase A
Gelatinase B
Hemorrhage
Histology
Humans
Immunohistochemistry
Interstitial collagenase
Laboratories
Leukocytes (neutrophilic)
Macrophages
Matrilysin
Matrix metalloproteinase
Matrix metalloproteinases
Matrix Metalloproteinases - metabolism
Medical research
Medical schools
Medicine
Membranes
Monocytes
Multiplexing
Neovascularization, Pathologic - complications
Neutrophil collagenase
Neutrophils
Patients
Quantitation
Retinopathy
Vascular endothelial growth factor
Vascular Endothelial Growth Factor A - metabolism
Vitreous Body - enzymology
Vitreous Body - metabolism
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Summary:To investigate which matrix metalloproteinases (MMPs) are more likely to be involved in the angiogenic process in proliferative diabetic retinopathy (PDR), we measured the levels of MMPs in the vitreous fluid from patients with PDR and controls and correlated these levels with the levels of vascular endothelial growth factor (VEGF). Vitreous samples from 32 PDR and 24 nondiabetic patients were studied by mosaic multiplex MMPs enzyme-linked immunosorbent assay (ELISA), single ELISA, Western blot and zymography analysis. Epiretinal membranes from 11 patients with PDR were studied by immunohistochemistry. MMP-8 and MMP-13 were not detected. ELISA, Western blot and gelatin ymography assays revealed significant increases in the expression levels of MMP-1, MMP-7, MMP-9 and VEGF in vitreous samples from PDR patients compared to nondiabetic controls, whereas MMP-2 and MMP-3 were not upregulated in vitreous samples from PDR patients. Significant correlations existed between ELISA and zymography assays for the quantitation of MMP-2 (r=0.407; p=0.039) and MMP-9 (r=0.711; p
ISSN:1932-6203
1932-6203
DOI:10.1371/journal.pone.0085857