Effect of Sfrp5 on cytokine release and insulin action in primary human adipocytes and skeletal muscle cells

• Published in: PloS one 2014-01, Vol.9 (1), p.e85906-e85906
• Main Authors: Carstensen, Maren, Wiza, Claudia, Röhrig, Karin, Fahlbusch, Pia, Roden, Michael, Herder, Christian, Ouwens, D Margriet
• Format: Article
• Language: English
• Subjects: Adipocytes; Adipocytes - drug effects; Adipocytes - metabolism; Adipocytes - secretion; Adiponectin; Adolescent; Adult; AKT protein; Analysis; Animals; Biochemistry; Biology; Cell culture; Cells, Cultured; Chemokines; Cytokines; Cytokines - secretion; Diabetes; DNA methylation; Endocrinology; Eye Proteins - metabolism; Eye Proteins - pharmacology; Female; Frizzled protein; Frizzled-related protein; Gene expression; Glucose; Glucose - metabolism; Humans; Inflammation; Inflammation - metabolism; Inflammation - pathology; Insulin; Insulin - metabolism; Insulin resistance; Interleukin 6; Interleukin 8; Interleukins; JNK protein; Kinases; Male; Medicine; Membrane Proteins - metabolism; Membrane Proteins - pharmacology; Metabolism; Mice; Middle Aged; Monocyte chemoattractant protein; Monocyte chemoattractant protein 1; Muscle Cells - drug effects; Muscle Cells - metabolism; Muscle, Skeletal - cytology; Muscles; Musculoskeletal system; NF-κB protein; Nutrition research; Obesity; Oxidative stress; Phosphorylation; Proteins; Proto-Oncogene Proteins - metabolism; R&D; Research & development; Sensitizing; Signal Transduction - drug effects; Signaling; Skeletal muscle; Studies; Tumor necrosis factor; Tumor Necrosis Factor-alpha - pharmacology; Tumor necrosis factor-α; Type 2 diabetes; White people; Wnt Proteins - metabolism; Wnt-5a Protein; Young Adult; Zebrafish
• ISSN: 1932-6203; 1932-6203
• DOI: 10.1371/journal.pone.0085906

Secreted frizzled-related protein 5 (Sfrp5) is an adipokine with anti-inflammatory and insulin-sensitizing properties in mice. However, the mechanism of Sfrp5 action, especially in humans, is largely unknown. Therefore, cytokine release and insulin signaling were analyzed to investigate the impact of Sfrp5 on inflammation and insulin signaling in primary human adipocytes and skeletal muscle cells (hSkMC). Sfrp5 neither affected interleukin (IL)-6, monocyte chemoattractant protein-1 (MCP-1) and adiponectin release from human adipocytes, nor IL-6 and IL-8 release from hSkMC. In tumor necrosis factor (TNF) α-treated adipocytes, Sfrp5 reduced IL-6 release by 49% (p