Patterning the cone mosaic array in zebrafish retina requires specification of ultraviolet-sensitive cones

• Published in: PloS one 2014-01, Vol.9 (1), p.e85325
• Main Authors: Raymond, Pamela A, Colvin, Steven M, Jabeen, Zahera, Nagashima, Mikiko, Barthel, Linda K, Hadidjojo, Jeremy, Popova, Lilia, Pejaver, Vivek R, Lubensky, David K
• Format: Article
• Language: English
• Subjects: Analysis; Animals; Biology; Cell Adhesion; Cell Communication - radiation effects; Cell Differentiation; Cell Polarity - radiation effects; Computer Science; Cones; Danio rerio; Developmental biology; Embryo, Nonmammalian; Epithelium; Gene Deletion; Gene Expression Regulation, Developmental; Genes; Genetic aspects; Image analysis; Image processing; In Situ Hybridization; Mathematical models; Membrane Proteins - genetics; Membrane Proteins - metabolism; Models, Biological; Morphogenesis - genetics; Patterning; Photoreception; Photoreceptors; Physics; Polarity; Proteins; Retina; Retinal Cone Photoreceptor Cells - metabolism; Retinal Cone Photoreceptor Cells - radiation effects; Retinal Cone Photoreceptor Cells - ultrastructure; Retinal Rod Photoreceptor Cells - metabolism; Retinal Rod Photoreceptor Cells - radiation effects; Retinal Rod Photoreceptor Cells - ultrastructure; Science; Signal Transduction; T-Box Domain Proteins - deficiency; T-Box Domain Proteins - genetics; Transcription (Genetics); Ultraviolet Rays; Vertebrates; Wavelengths; Zebrafish; Zebrafish - embryology; Zebrafish - genetics; Zebrafish - metabolism; Zebrafish Proteins - deficiency; Zebrafish Proteins - genetics; Zebrafish Proteins - metabolism
• ISSN: 1932-6203; 1932-6203
• DOI: 10.1371/journal.pone.0085325

Cone photoreceptors in teleost fish are organized in precise, crystalline arrays in the epithelial plane of the retina. In zebrafish, four distinct morphological/spectral cone types occupy specific, invariant positions within a regular lattice. The cone lattice is aligned orthogonal and parallel to circumference of the retinal hemisphere: it emerges as cones generated in a germinal zone at the retinal periphery are incorporated as single-cell columns into the cone lattice. Genetic disruption of the transcription factor Tbx2b eliminates most of the cone subtype maximally sensitive to ultraviolet (UV) wavelengths and also perturbs the long-range organization of the cone lattice. In the tbx2b mutant, the other three cone types (red, green, and blue cones) are specified in the correct proportion, differentiate normally, and acquire normal, planar polarized adhesive interactions mediated by Crumbs 2a and Crumbs 2b. Quantitative image analysis of cell adjacency revealed that the cones in the tbx2b mutant primarily have two nearest neighbors and align in single-cell-wide column fragments that are separated by rod photoreceptors. Some UV cones differentiate at the dorsal retinal margin in the tbx2b mutant, although they are severely dysmorphic and are eventually eliminated. Incorporating loss of UV cones during formation of cone columns at the margin into our previously published mathematical model of zebrafish cone mosaic formation (which uses bidirectional interactions between planar cell polarity proteins and anisotropic mechanical stresses in the plane of the retinal epithelium to generate regular columns of cones parallel to the margin) reproduces many features of the pattern disruptions seen in the tbx2b mutant.