Microbial diversity of Emalahleni mine water in South Africa and tolerance ability of the predominant organism to vanadium and nickel

The present study aims firstly at determining the microbial diversity of mine-water collected in Emalahleni, South Africa and secondly isolating and characterizing the most dominant bacterial species found in the mine water in terms of its resistance to both V(5+) and Ni(2+) in a modified wastewater...

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Bibliographic Details
Published in:PloS one 2014-01, Vol.9 (1), p.e86189
Main Authors: Kamika, Ilunga, Momba, Maggie N B
Format: Article
Language:English
Subjects:
Acid mine drainage
Adaptation, Biological
Anabaena
Bacteria
Biodiversity
Biofilms
Biology
Bioremediation
Chemical elements
Chemical oxygen demand
Chemistry
Deoxyribonucleic acid
DNA
Earth science
Genetic testing
Heavy metals
Humans
Hydrogen-Ion Concentration
Industrial Waste
Metagenome
Metals
Microbiota
Microorganisms
Mine drainage
Mine water
Minimum inhibitory concentration
Molecular Sequence Data
Nickel
Nickel - toxicity
pH effects
Phylogeny
Pollution
RNA, Ribosomal, 16S
South Africa
Vanadium
Vanadium - toxicity
Waste Water - microbiology
Wastewater
Water analysis
Water Microbiology
Water pollution
Water sampling
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Summary:The present study aims firstly at determining the microbial diversity of mine-water collected in Emalahleni, South Africa and secondly isolating and characterizing the most dominant bacterial species found in the mine water in terms of its resistance to both V(5+) and Ni(2+) in a modified wastewater liquid media. The results revealed a microbial diversity of 17 orders, 27 families and 33 genera were found in the mine-water samples with Marinobacteria (47.02%) and Anabaena (17.66%) being the most abundant genera. Considering their abundance in the mine-water samples, a species of the Marinobacter genera was isolated, identified, and characterised for metal tolerance and removal ability. The MWI-1 isolate (Marinobacter sp. MWI-1 [AB793286]) was found to be closely related to Marinobacter goseongensis at 97% of similarity. The isolate was exposed to various concentrations of Ni(2+) and V(5+) in wastewater liquid media and its tolerance to metals was also assessed. The MWI-1 isolate could tolerate V(5+) and Ni(2+) separately at concentrations (in terms of MIC) up to 13.41 ± 0.56 mM and 5.39 ± 0.5 mM at pH 7, whereas at pH 3, the tolerance limit decrease to 11.45 ± 0.57 mM and 2.67 ± 0.1 mM, respectively. The removal of V(5+) and Ni(2+) in liquid media was noted to gradually decrease with a gradual increase of the test metals. A significant difference (p
ISSN:1932-6203
1932-6203
DOI:10.1371/journal.pone.0086189