Importance of the CEP215-pericentrin interaction for centrosome maturation during mitosis

At the onset of mitosis, the centrosome undergoes maturation, which is characterized by a drastic expansion of the pericentriolar material (PCM) and a robust increase in microtubule-organizing activity. CEP215 is one of the major PCM components which accumulates at the centrosome during mitosis. The...

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Bibliographic Details
Published in:PloS one 2014-01, Vol.9 (1), p.e87016-e87016
Main Authors: Kim, Seongjae, Rhee, Kunsoo
Format: Article
Language:English
Subjects:
Antigens - chemistry
Antigens - metabolism
Biological effects
Biology
Cell cycle
Centrosome - metabolism
Centrosome - physiology
HEK293 Cells
HeLa Cells
Humans
Immunoblotting
Immunohistochemistry
Immunoprecipitation
Intracellular Signaling Peptides and Proteins - metabolism
Localization
Maturation
Microcephaly
Microscopy, Fluorescence
Mitosis
Mitosis - physiology
Models, Molecular
Mutation
Nerve Tissue Proteins - metabolism
Protein interaction
Protein-protein interactions
Proteins
Recruitment
RNA, Small Interfering - genetics
Spindle Apparatus - genetics
Spindle Apparatus - physiology
Tubulin
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Summary:At the onset of mitosis, the centrosome undergoes maturation, which is characterized by a drastic expansion of the pericentriolar material (PCM) and a robust increase in microtubule-organizing activity. CEP215 is one of the major PCM components which accumulates at the centrosome during mitosis. The depletion phenotypes indicate that CEP215 is essential for centrosome maturation and bipolar spindle formation. Here, we performed a series of knockdown-rescue experiments to link the protein-protein interaction properties of CEP215 to its biological functions. The results showed that CEP215 and pericentrin, another major PCM component, is interdependent for their accumulation at the spindle poles during mitosis. As a result, The CEP215-pericentrin interaction is required for centrosome maturation and subsequent bipolar spindle formation during mitosis. On the other hand, CEP215 interaction with γ-tubulin is dispensable for centrosome maturation. Our results provide an insight how PCM components are assembled to form a spindle pole during mitosis.
ISSN:1932-6203
1932-6203
DOI:10.1371/journal.pone.0087016