Equations for lipid normalization of carbon stable isotope ratios in aquatic bird eggs

• Published in: PloS one 2014-01, Vol.9 (1), p.e83597-e83597
• Main Authors: Elliott, Kyle H, Davis, Mikaela, Elliott, John E
• Format: Article
• Language: English
• Subjects: Algebra; Algorithms; Analysis; Animals; Aquatic birds; Biology; Bird eggs; Birds; Birds - classification; Birds - metabolism; Carbon; Carbon 13; Carbon isotopes; Carbon Isotopes - analysis; Chemical analysis; Chemistry; Chemistry Techniques, Analytical - methods; Coastal ecology; Contaminants; Earth Sciences; Ecosystem; Eggs; Fatty acids; Fresh Water; Isotope ratios; Isotopes; Linear equations; Lipid Metabolism; Lipids; Lipids - analysis; Mathematical analysis; Mathematical models; Mathematics; Migratory species; Muscles; Nitrogen; Nitrogen (Chemical element); Nitrogen Isotopes - metabolism; Nutrients; Ovum - chemistry; Proteins; Ratios; Reproducibility of Results; Seawater; Species Specificity; Sulfur; Sulfur compounds; Sulfur isotopes; Terrestrial environments; Tracers
• ISSN: 1932-6203; 1932-6203
• DOI: 10.1371/journal.pone.0083597

Stable isotope ratios are biogeochemical tracers that can be used to determine the source of nutrients and contaminants in avian eggs. However, the interpretation of stable carbon ratios in lipid-rich eggs is complicated because (13)C is depleted in lipids. Variation in (13)C abundance can therefore be obscured by variation in percent lipids. Past attempts to establish an algebraic equation to correct carbon isotope ratios for lipid content in eggs have been unsuccessful, possibly because they relied partly on data from coastal or migratory species that may obtain egg lipids from different habitats than egg protein. We measured carbon, nitrogen and sulphur stable isotope ratios in 175 eggs from eight species of aquatic birds. Carbon, nitrogen and sulphur isotopes were enriched in lipid-extracted egg samples compared with non extracted egg samples. A logarithmic equation using the C∶N ratio and carbon isotope ratio from the non extracted egg tissue calculated 90% of the lipid-extracted carbon isotope ratios within ±0.5‰. Calculating separate equations for eggs laid by species in different habitats (pelagic, offshore and terrestrial-influenced) improved the fit. A logarithmic equation, rather than a linear equation as often used for muscle, was necessary to accurately correct for lipid content because the relatively high lipid content of eggs compared with muscle meant that a linear relationship did not accurately approximate the relationship between percent lipids and the C∶N ratio. Because lipid extraction alters sulphur and nitrogen isotope ratios (and cannot be corrected algebraically), we suggest that isotopic measurement on bulk tissue followed by algebraic lipid normalization of carbon stable isotope ratio is often a good solution for homogenated eggs, at least when it is not possible to complete separate chemical analyses for each isotope.