Caveolin-1 provides palliation for adverse hepatic reactions in hypercholesterolemic rabbits

• Published in: PloS one 2014-01, Vol.9 (1), p.e71862-e71862
• Main Authors: Chen, Ya-Hui, Lin, Wei-Wen, Liu, Chin-San, Hsu, Li-Sung, Lin, Yueh-Min, Su, Shih-Li
• Format: Article
• Language: English
• Subjects: Animals; Antioxidants; Apoptosis; Apoptosis - physiology; Atherosclerosis; Biochemistry; Biology; Biosynthesis; Catalase; Caveolae; Caveolin; Caveolin 1 - metabolism; Caveolin-1; Cell death; Cellular signal transduction; Cellular structure; Chains; Cholesterol; Cristae; Deoxyribonucleic acid; Diabetes; Diabetic neuropathy; Diabetic retinopathy; Diet; Diet, Atherogenic; DNA; Electron Transport; Enzymes; Gene expression; Hepatocytes; Hepatocytes - metabolism; Homeostasis; Hospitals; Hypercholesterolemia; Hypercholesterolemia - blood; Hypercholesterolemia - metabolism; Hypertension; Immunohistochemistry; Intravenous administration; Invaginations; Lipid Metabolism; Lipids; Lipoproteins; Liver; Liver - metabolism; Liver - pathology; Low density lipoprotein; Low density lipoproteins; Male; Malondialdehyde; Medicine; Membrane Proteins - metabolism; Mitochondria; Mitochondria - metabolism; Mitochondrial DNA; Nitric oxide; Nuclear reactions; Organs; Oxidative Stress; Palliation; Peptides; Proteins; Pulmonary arteries; Rabbits; Reactive oxygen species; Reactive Oxygen Species - metabolism; Rodents; Signal processing; Signal transduction; Stress concentration; Studies; Superoxide dismutase; Superoxides; Tissue analysis
• ISSN: 1932-6203; 1932-6203
• DOI: 10.1371/journal.pone.0071862

Caveolins are an essential component of cholesterol-rich invaginations of the plasma membrane known as caveolae. These flask-shaped, invaginated structures participate in a number of important cellular processes, including vesicular transport, cholesterol homeostasis, and signal transduction. We investigated the effects of CAV-1 on mitochondrial biogenesis and antioxidant enzymes in hypercholesterolemia-affected target organs. A total of eighteen male New Zealand white rabbits were divided into three groups: a normal-diet group, an untreated hypercholesterolemia-induced group, and a hypercholesterolemia-induced group that received intravenous administration of antennapedia-CAV-1 (AP-CAV-1) peptide every 2 days for 2 weeks. Serum biochemistry, CAV-1 distribution, neutral lipid distribution, mitochondrial morphology, biogenesis-mediated protein content, oxidative stress balance, antioxidant enzyme levels, and apoptotic cell death of liver tissue were analysed. Hepatic and circulating cholesterol and low-density lipoprotein cholesterol (LDL-C) levels differed significantly between the three groups (P